Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-PZR (Tyr263) Antibody #5543

No. Size Price
5543S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价
5543 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Bovine, Endogenous 30-50 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-PZR (Tyr263) Antibody recognizes endogenous levels of PZR protein only when phosphorylated at Tyr263. This antibody does not cross-react with other tyrosine kinase proteins.

Phospho-PZR (Tyr263)抗体仅可以识别263酪氨酸磷酸化后后的PZR蛋白。抗体与其他酪氨酸激酶蛋白没有交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr263 of human PZR protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from BAEC cells, untreated or H2O2-treated (0.3 mM for 30 min), using Phospho-PZR (Tyr263) Antibody (upper) and total PZR Antibody # 4157 (lower).未处理货经过H2O2处理(0.3 mM 30分钟)的BAEC细胞提取物进行western blot分析,使用Phospho-PZR (Tyr263)抗体(上)和总PZR抗体#4157(下)进行试验。


PZR (Protein zero related) is an immunoglobulin superfamily protein that specifically binds the tyrosine phosphatase SHP-2 through its intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1,2). PZR is phosphorylated by c-Src, c-Fyn, c-Lyn, Csk, and c-Abl (3). PP1, a Src family kinase inhibitor, inhibits PZR phosphorylation (4,5). There are three alternatively spliced isoforms, designated as PZR, PZRa, and PZRb; both PZRa and PZRb lack ITIMs (6,7). PZR is the main receptor of ConA and has an important role in cell signaling via c-Src (4). PZR is expressed in many cell types and is localized to cell contacts and intracellular granules in BAECs and mesothelioma (REN) cells. PZR has been implicated as a cell adhesion protein that may be involved in SHP-2-dependent signaling at interendothelial cell contacts (3). Hypertyrosine phosphorylation of PZR was observed during embryogenesis in a mouse model of Noonan syndrome (8).

PZR(蛋白zero相关)是一个免疫球超家族蛋白可以通过其细胞内免疫受体:基于酪氨酸的抑制基序(ITIMs)与酪氨酸磷酸酯酶SHP-2特异性结合(1,2)。PZR可以被c-Src, c-Fyn, c-Lyn, Csk, 和c-Abl磷酸化(3)。PP1,一个Src家族激酶抑制子,能够抑制PZR磷酸化(4,5)。PZR有3个剪切异构体,分别命名为PZR, PZRa, and PZRb;PZRa和PZRb都缺乏ITIMs(6,7)。PZR是刀豆素A的主要受体在细胞信号传导中通过c-Src发挥重要作用(4)。PZR在多种细胞中表达,位于BAECs和间皮瘤(REN)细胞连接和细胞内颗粒中。PZR被认为是一种细胞粘附分子,可能在SHP-2-依赖的信号通路中涉及内皮细胞间连接(3)。Noonan 综合征小鼠模型的胚胎发育时期可以观察到PZR酪氨酸高度磷酸化(8)。

  1. Zhao, Z.J. and Zhao, R. (1998) J Biol Chem 273, 29367-72.
  2. Zhao, R. and Zhao, Z.J. (2000) J Biol Chem 275, 5453-9.
  3. Kusano, K. et al. Endothelium 15, 127-36.
  4. Zhao, R. et al. (2002) J Biol Chem 277, 7882-8.
  5. Zhao, R. et al. (2003) J Biol Chem 278, 42893-8.
  6. Zannettino, A.C. et al. (2003) Biochem J 370, 537-49.
  7. Zhao, R. and Zhao, Z.J. (2003) Biochem Biophys Res Commun 303, 1028-33.
  8. Eminaga, S. and Bennett, A.M. (2008) J Biol Chem 283, 15328-38.

Application References

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