Cell Signaling Technology

Product Pathways - Protein Translation

HIF-1β/ARNT (D28F3) XP® Rabbit mAb #5537

ARNT   Aryl hydrocarbon receptor nuclear translocator (ARNT)   HIF   HIF-1   HIF-1b   HIF-1beta   HIF1   HIF1b   HIF1beta   Hypoxia   Hypoxia-inducible factor 1 beta  

No. Size Price
5537S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
5537T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
5537 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 87 Rabbit IgG
IP 1:50
IHC-P 1:100
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), ChIP=Chromatin IP,

Specificity / Sensitivity

HIF-1β/ARNT (D28F3) XP® Rabbit mAb detects endogenous levels of total HIF-1β/ARNT protein.

HIF-1β/ARNT (D28F3) XP® Rabbit mAb兔单抗识别内源性的HIF-1β/ARNT 总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence around Ile479 of human HIF-1β/ARNT protein.

单克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与包围于HIF-1β/ARNT蛋白异亮氨酸479附近残基相对应。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using HIF-1β/ARNT (D28F3) XP® Rabbit mAb.使用HIF-1β/ARNT (D28F3) XP® 兔单抗对多种细胞提取物进行western blot实验。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using HIF-1β/ARNT (D28F3) XP® Rabbit mAb.使用HIF-1β/ARNT (D28F3) XP® 兔单抗对石蜡包埋的人肺癌组织进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HIF-1β/ARNT (D28F3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).在对照多肽(左)或抗原特异性多肽(右)存在的条件下使用HIF-1β/ARNT (D28F3) XP®兔单抗对石蜡包埋的人乳腺癌组织进行免疫组化实验。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse colon using HIF-1β/ARNT (D28F3) XP® Rabbit mAb.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 T47D cells treated with BNF (1μM) for 45 min and either 10 μl of HIF-1β/ARNT (D28F3) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human NFE2L2 Intron 1 Primers #81126, human CYP1A1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism, and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).

HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8). In addition, a chromosomal translocation leading to a TEL-ARNT fusion protein is associated with acute myeloblastic leukemia (9). Studies also found that ARNT/HIF-1β expression levels decrease significantly in pancreatic islets from patients with type 2 diabetes, suggesting that HIF-1β plays an important role in pancreatic β-cell function (10).

缺氧诱导因子1(HIF1)是一个二聚体转录因子在细胞应激缺氧环境时会发挥作用(1)。HIF1复合物包括两个亚基,HIF-1α和HIF-1β,都属于PAS(Per, ARNT, Sim)碱性螺旋-环-螺旋家族(2)。HIF1调控多种应激缺氧环境的基因,包括调控血管生成,红细胞生成,细胞周期,细胞代谢和细胞凋亡过程的基因。广泛表达的HIF-1α通常是由泛素/蛋白酶体途径迅速在含氧量正常的细胞中降解的。在常氧条件下,HIF-1α被脯氨酸羟基化促进了其被绑定到von Hippel Lindau蛋白(VLH)E3连接酶复合物引起构象变化,随后被泛素化和蛋白酶体降解(3,4)。低氧条件和化学羟化酶抑制剂(如去铁胺和钴)抑制HIF-1α的降解,并增加其蛋白稳定性。此外,HIF-1α可通过各种细胞因子经过PI3K-AKT-mTOR通路诱导进入氧无关的信号通路(5-7)。

HIF-1β由于能够以伴侣方式与芳香烃受体(AHR)结合形成异二聚体复合物,也被称为芳香烃受体核转位(ARNT) (8)。HIF-1β与AhR能够在外源性化学物质代谢中发挥重要作用(8)。除此以外染色体转位导致的TEL-ARNT融合蛋白和急性髓细胞白血病相关(9)。研究也发现ARNT/HIF-1β的表达水平在2型糖尿病人的胰岛内明显表达下降,提示HIF-1β在胰岛β细胞功能过程中发挥作用(10)。

  1. Sharp, F.R. and Bernaudin, M. (2004) Nat Rev Neurosci 5, 437-48.
  2. Wang, G.L. et al. (1995) Proc Natl Acad Sci U S A 92, 5510-4.
  3. Jaakkola, P. et al. (2001) Science 292, 468-72.
  4. Maxwell, P.H. et al. (1999) Nature 399, 271-5.
  5. Fukuda, R. et al. (2002) J Biol Chem 277, 38205-11.
  6. Jiang, B.H. et al. (2001) Cell Growth Differ 12, 363-9.
  7. Laughner, E. et al. (2001) Mol Cell Biol 21, 3995-4004.
  8. Walisser, J.A. et al. (2004) Proc Natl Acad Sci U S A 101, 16677-82.
  9. Salomon-Nguyen, F. et al. (2000) Proc Natl Acad Sci U S A 97, 6757-62.
  10. Gunton, J.E. et al. (2005) Cell 122, 337-49.

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