Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb #5536

FK506 binding protein 12-rapamycin associated protein 1   FRAP1   Mammalian target of rapamycin   mTOR   pmtor   rapamycin   Rapamycin target protein (RAPT1)   RAPT   RAPT1   TOR  

No. Size Price
5536S 100 µl ( 10 western blots ) ¥3,990.00 现货查询 购买询价
5536T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价
5536 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 289 Rabbit IgG
IP 1:50
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Rat, Chicken, Pig, Horse,

Specificity / Sensitivity

Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb detects endogenous levels of mTOR protein only when phosphorylated at Ser2448.

Phospho-mTOR (Ser2448) (D9C2) XP ® Rabbit mAb兔单抗可以检测出在Ser2448位点磷酸化的内源性总mTOR蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser2448 of human mTOR protein.




Confocal immunofluorescent analysis of HeLa cells, rapamycin-treated (#9904, 10 μM for 2 hours, left), insulin-treated (150 nM for 6 minutes, middle) or insulin- and λ-phosphatase-treated (right), using Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析HeLa 细胞, rapamycin处理 (#9904, 10 μM for 2 hours, 左),胰岛素处理(150 nM for 6 minutes, 中) 或胰岛素和 λ磷酸酶处理(右), 所用抗体为Phospho-mTOR (Ser2448) (D9C2) XP ® Rabbit mAb (绿色)。肌动蛋白丝采用DY-554 phalloidin标记。蓝色伪彩是DRAQ5 ® #4084 (荧光 DNA 染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved NIH/3T3 cells, untreated or insulin-treated (150 nM, 5 minutes), alone or in combination with λ-phosphatase, using Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb (upper) or mTOR (7C10) Rabbit mAb #2983.

Western blot 分析血清饥饿的NIH/3T3细胞提取物,未处理组和胰岛素(150 nM, 5 minutes)单独或联合λ-磷酸酶处理组,所用抗体为Phospho-mTOR (Ser2448) (D9C2) XP ® Rabbit mAb 兔单抗(upper) 或 mTOR (7C10) Rabbit mAb 兔单抗#2983。


The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).

哺乳动物雷帕霉素(rapamycin)靶蛋白(mTOR,FRAP,RAFT)是一种丝氨酸/苏氨酸蛋白激酶(1-3),其作为ATP和氨基酸的传感器,可以有效调控营养供应及细胞的生长(4,5)。当营养充分时,mTOR响应磷脂酸介导的信号,进而正向调节p70 S6 激酶,并参与失活eIF4E的抑制剂4E-BP1。这些信号导致特定的mRNA亚群的翻译。mTOR可以被Akt信号通路的PI3K激酶磷酸化修饰Ser2448位点,也能自我磷酸化Ser2481位点(7,8)。mTOR的在细胞生长和动态平衡中起着关键作用,在肿瘤中可能存在调控失常。因此,目前很多研究将mTOR作为一个潜在的抗肿瘤治疗靶点(9)。

  1. Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22.
  2. Brown, E.J. et al. (1994) Nature 369, 756-8.
  3. Sabatini, D.M. et al. (1994) Cell 78, 35-43.
  4. Gingras, A.C. et al. (2001) Genes Dev 15, 807-26.
  5. Dennis, P.B. et al. (2001) Science 294, 1102-5.
  6. Fang, Y. et al. (2001) Science 294, 1942-5.
  7. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
  8. Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23.
  9. Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.

Application References

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