Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb #5536

FK506 binding protein 12-rapamycin associated protein 1   FRAP1   Mammalian target of rapamycin   mTOR   pmtor   rapamycin   Rapamycin target protein (RAPT1)   RAPT   RAPT1   TOR  

No. Size Price
5536S 100 µl ( 10 western blots ) ¥4,180.00 现货查询 购买询价 防伪查询
5536T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价 防伪查询
5536 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 289 Rabbit IgG
IP 1:50
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Rat, Chicken, Pig, Horse,

Specificity / Sensitivity

Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb detects endogenous levels of mTOR protein only when phosphorylated at Ser2448.

Phospho-mTOR (Ser2448) (D9C2) XP ® Rabbit mAb兔单抗可以检测出在Ser2448位点磷酸化的内源性总mTOR蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser2448 of human mTOR protein.




Confocal immunofluorescent analysis of HeLa cells, rapamycin-treated (#9904, 10 μM for 2 hours, left), insulin-treated (150 nM for 6 minutes, middle) or insulin- and λ-phosphatase-treated (right), using Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析HeLa 细胞, rapamycin处理 (#9904, 10 μM for 2 hours, 左),胰岛素处理(150 nM for 6 minutes, 中) 或胰岛素和 λ磷酸酶处理(右), 所用抗体为Phospho-mTOR (Ser2448) (D9C2) XP ® Rabbit mAb (绿色)。肌动蛋白丝采用DY-554 phalloidin标记。蓝色伪彩是DRAQ5 ® #4084 (荧光 DNA 染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved NIH/3T3 cells, untreated or insulin-treated (150 nM, 5 minutes), alone or in combination with λ-phosphatase, using Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb (upper) or mTOR (7C10) Rabbit mAb #2983.

Western blot 分析血清饥饿的NIH/3T3细胞提取物,未处理组和胰岛素(150 nM, 5 minutes)单独或联合λ-磷酸酶处理组,所用抗体为Phospho-mTOR (Ser2448) (D9C2) XP ® Rabbit mAb 兔单抗(upper) 或 mTOR (7C10) Rabbit mAb 兔单抗#2983。


The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).

哺乳动物雷帕霉素(rapamycin)靶蛋白(mTOR,FRAP,RAFT)是一种丝氨酸/苏氨酸蛋白激酶(1-3),其作为ATP和氨基酸的传感器,可以有效调控营养供应及细胞的生长(4,5)。当营养充分时,mTOR响应磷脂酸介导的信号,进而正向调节p70 S6 激酶,并参与失活eIF4E的抑制剂4E-BP1。这些信号导致特定的mRNA亚群的翻译。mTOR可以被Akt信号通路的PI3K激酶磷酸化修饰Ser2448位点,也能自我磷酸化Ser2481位点(7,8)。mTOR的在细胞生长和动态平衡中起着关键作用,在肿瘤中可能存在调控失常。因此,目前很多研究将mTOR作为一个潜在的抗肿瘤治疗靶点(9)。

  1. Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22.
  2. Brown, E.J. et al. (1994) Nature 369, 756-8.
  3. Sabatini, D.M. et al. (1994) Cell 78, 35-43.
  4. Gingras, A.C. et al. (2001) Genes Dev 15, 807-26.
  5. Dennis, P.B. et al. (2001) Science 294, 1102-5.
  6. Fang, Y. et al. (2001) Science 294, 1942-5.
  7. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
  8. Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23.
  9. Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :