Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose® Bead Conjugate) #5501

No. Size Price
5501S 400 µl ( 40 immunoprecipitations ) ¥3,986.00 现货查询 购买询价
5501 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IP 1:20 All Species Expected, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate) detects phospho-serine in a PXS*P or S*PXR/K motif, as well as a PXS*PXR/K motif. The antibody is phospho-specific, and does not react with phospho-threonine- or phospho-tyrosine-containing peptides/proteins. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate)兔单抗能够检测PXS*P或S*PXR/K基序,以及PXS*PXR/K基序中的磷酸化丝氨酸。此抗体是磷酸化特异性的,与包含磷酸化苏氨酸或磷酸化酪氨酸的肽段或蛋白无交叉反应。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及其国外相应专利)

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-MAPK/CDK substrate peptides.

该单克隆抗体用合成的磷酸化MAPK/CDK底物多肽免疫动物制备。

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K). The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325.

该CST抗体是通过主要的氨基酸集团共价结合N-羟基丁二酰亚胺(NHS)活化的琼脂糖珠进行固定的。Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate)兔单抗对于Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K)的免疫沉淀非常有用。该抗体与未偶联的Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb兔单抗 #2325具有相同的种属交叉反应性。

IP

IP

Immunoprecipitation of Jurkat cell extracts, untreated (-) or treated with Calyculin A #9902 (+), using Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate) and Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423. The western blot was probed using Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.

免疫沉淀法检测未经处理(-)或Calyculin A #9902处理(+) 的Jurkat细胞提取物,使用抗体为Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb (Sepharose Bead Conjugate) 以及 Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423。Western blot 检测中使用的是Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325。使用的二抗是Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127。

Background

The MAPK and CDK families of serine/threonine protein kinases play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-3). MAPK phosphorylates substrates with the consensus sequence PX(S/T)P, and CDKs phosphorylate substrates containing the consensus sequence (S/T)PXR/K. Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline, motifs PXS*/T*P and/or S*PXR/K, for use in the study and discovery of new MAPK and CDK substrates (4,5).

MAPK和CDK丝氨酸/苏氨酸蛋白激酶家族在细胞增殖和周期控制中扮演重要角色。这些激酶磷酸化脯氨酸残基前的苏氨酸或丝氨酸(1-3)。MAPK磷酸化含有共有序列PX(S/T)P的底物,CDKs磷酸化含有共有序列(S/T)PXK/R的底物。CST公司开发了可以结合脯氨酸残基前的磷酸化苏氨酸、基序为PXS*/T*P和或S*PXK/R的抗体,用以研究和发现新的MAPK和CDK底物(4,5)。

  1. Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
  2. Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.
  3. Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
  4. Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6.
  5. Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93.

Application References

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Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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