Cell Signaling Technology

Product Pathways - Adhesion

Phospho-Afadin (Ser1718) Antibody #5485

adherens junctions   AF6   ALL1-fused gene from chromosome 6 protein   MLLT4   tight junctions  

No. Size Price
5485S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5485 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 205 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Dog,

Specificity / Sensitivity

Phospho-Afadin (Ser1718) Antibody detects endogenous levels of l-afadin protein only when phosphorylated at serine 1718.

Phospho-Afadin (Ser1718)抗体仅能识别1718位丝氨酸磷酸化后的内源性1-afadin蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1718 of human afadin. Antibodies are purified using protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or EGF-treated (100 ng/ml for 5 minutes), using Phospho-Afadin (Ser1718) Antibody.对未处理货经过EGF-处理(100 ng/ml 5分钟)对A431细胞提取物进行western blot分析,使用Phospho-Afadin (Ser1718) 抗体。


In multicellular organisms, intercellular junctions play essential roles in tissue integrity and maintenance of cell polarity. Tight junctions (TJs) form a continuous barrier to fluids across the epithelium and endothelium (reviewed in 1). Adherens junctions (AJs) are dynamic structures that form cell-cell contacts linking cells into a continuous sheet (reviewed in 2). The actin filament-binding protein, Afadin, binds to nectin forming a connection to the action cytoskeleton (3). AJs are formed when nectin assembles cadherin at the cell-cell adhesion site and these junctions are then involved in the formation and maintenance of TJs (4, 5). Afadin has two splice variants: l-afadin, which is ubiquitously expressed, and s-afadin, which is expressed predominantly in neural tissue. s-Afadin is a shorter form lacking one of the three proline-rich regions found in l-afadin as well the carboxyl-terminal F-actin binding region (6). Human s-afadin is identical to AF-6, the ALL-1 fusion partner involved in acute myeloid leukemias (7). Recent work has also shown that afadin is involved in controlling the directionality of cell movement when it is localized at the leading edge of moving cells (8, 9).


Phospho-Afadin (Ser1718) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser1718 was discovered using an Akt substrate antibody. Please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org for more information.

Cell Signaling Technology (CST) 使用PTMScan®识别的位点以Phospho-Afadin (Ser1718)抗体,本公司LC-MS/MS平台用于发现修饰位点。1718位丝氨酸磷酸化通过Akt底物抗体发现。请访问PhosphoSitePlus®,一个由CST设计的修饰位点数据库,更多信息请访问www.phosphosite.org。

  1. Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
  2. Harris, T.J. and Tepass, U. (2010) Nat Rev Mol Cell Biol 11, 502-14.
  3. Ikeda, W. et al. (1999) J Cell Biol 146, 1117-32.
  4. Sato, T. et al. (2006) J Biol Chem 281, 5288-99.
  5. Ooshio, T. et al. (2007) J Cell Sci 120, 2352-65.
  6. Mandai, K. et al. (1997) J Cell Biol 139, 517-28.
  7. Prasad, R. et al. (1993) Cancer Res 53, 5624-8.
  8. Miyata, M. et al. (2009) J Cell Sci 122, 4319-29.
  9. Miyata, M. et al. (2009) J Biol Chem 284, 24595-609.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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