Cell Signaling Technology

Product Pathways - Adhesion

Phospho-Afadin (Ser1718) Antibody #5485

adherens junctions   AF6   ALL1-fused gene from chromosome 6 protein   MLLT4   tight junctions  

No. Size Price
5485S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5485 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 205 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Dog,

Specificity / Sensitivity

Phospho-Afadin (Ser1718) Antibody detects endogenous levels of l-afadin protein only when phosphorylated at serine 1718.

Phospho-Afadin (Ser1718)抗体仅能识别1718位丝氨酸磷酸化后的内源性1-afadin蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1718 of human afadin. Antibodies are purified using protein A and peptide affinity chromatography.

合成对应人afadin1718位丝氨酸及其邻近氨基酸残基序列的多肽免疫动物获得多克隆抗体。抗体通过蛋白A和肽亲和层析纯化获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or EGF-treated (100 ng/ml for 5 minutes), using Phospho-Afadin (Ser1718) Antibody.对未处理货经过EGF-处理(100 ng/ml 5分钟)对A431细胞提取物进行western blot分析,使用Phospho-Afadin (Ser1718) 抗体。

Background

In multicellular organisms, intercellular junctions play essential roles in tissue integrity and maintenance of cell polarity. Tight junctions (TJs) form a continuous barrier to fluids across the epithelium and endothelium (reviewed in 1). Adherens junctions (AJs) are dynamic structures that form cell-cell contacts linking cells into a continuous sheet (reviewed in 2). The actin filament-binding protein, Afadin, binds to nectin forming a connection to the action cytoskeleton (3). AJs are formed when nectin assembles cadherin at the cell-cell adhesion site and these junctions are then involved in the formation and maintenance of TJs (4, 5). Afadin has two splice variants: l-afadin, which is ubiquitously expressed, and s-afadin, which is expressed predominantly in neural tissue. s-Afadin is a shorter form lacking one of the three proline-rich regions found in l-afadin as well the carboxyl-terminal F-actin binding region (6). Human s-afadin is identical to AF-6, the ALL-1 fusion partner involved in acute myeloid leukemias (7). Recent work has also shown that afadin is involved in controlling the directionality of cell movement when it is localized at the leading edge of moving cells (8, 9).

在多细胞生物体内,细胞间连接对于组织整合和保持细胞极性有重要作用。紧密连接(TJs)在上皮和内皮件形成了一道连续阻碍液体的壁垒(见综述1)。粘附连接(AJs)是一种动态结构能够将细胞和细胞连接后结合到连续的层状结构上(见综述2)。肌动蛋白丝结合蛋白,Afadin,与结合素结合与肌动蛋白丝细胞骨架形成连接(3)。当结合素在细胞-细胞间连接位置富集钙粘结蛋白时会形成AJs,随后这种连接将参与形成和维持TJs(4,5)。Afadin有两种剪切异构体:广泛表达的1-afadin,主要表达于神经组织的s-afadin。S-Afadin是一种更短的形式,缺乏了1-afadin中三个富含脯氨酸区域的其中一个,以及羧基末端的F-肌动蛋白丝结合区域(6)。人s-afadin被认为是AF-6,ALL-1融合伴侣涉及急性髓性白血病(7)。最近的研究表明当细胞定位到运动的细胞边缘时,afadin涉及控制这些细胞运动的方向(8,9)。

Phospho-Afadin (Ser1718) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser1718 was discovered using an Akt substrate antibody. Please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org for more information.

Cell Signaling Technology (CST) 使用PTMScan®识别的位点以Phospho-Afadin (Ser1718)抗体,本公司LC-MS/MS平台用于发现修饰位点。1718位丝氨酸磷酸化通过Akt底物抗体发现。请访问PhosphoSitePlus®,一个由CST设计的修饰位点数据库,更多信息请访问www.phosphosite.org。

  1. Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
  2. Harris, T.J. and Tepass, U. (2010) Nat Rev Mol Cell Biol 11, 502-14.
  3. Ikeda, W. et al. (1999) J Cell Biol 146, 1117-32.
  4. Sato, T. et al. (2006) J Biol Chem 281, 5288-99.
  5. Ooshio, T. et al. (2007) J Cell Sci 120, 2352-65.
  6. Mandai, K. et al. (1997) J Cell Biol 139, 517-28.
  7. Prasad, R. et al. (1993) Cancer Res 53, 5624-8.
  8. Miyata, M. et al. (2009) J Cell Sci 122, 4319-29.
  9. Miyata, M. et al. (2009) J Biol Chem 284, 24595-609.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications