Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Ezrin (Tyr353) (D66D3) Rabbit mAb #5484

No. Size Price
5484S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5484 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 80 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Monkey, Dog,

Specificity / Sensitivity

Phospho-Ezrin (Tyr353) (D66D3) Rabbit mAb recognizes endogenous levels of ezrin protein only when phosphorylated at Tyr353. Based on protein sequences, the antibody is not expected to cross-react with radixin or moesin.

Phospho-Ezrin (Tyr353) (D66D3) Rabbit mAb仅识别Tyr353磷酸化的内源性ezrin蛋白。基于蛋白质序列比对预测此抗体不与radixin或moesin蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr353 of human ezrin protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated or treated with hEGF #8916 (100 ng/ml, 5 minutes), using Phospho-Ezrin (Tyr353) (D66D3) Rabbit mAb (upper) or Ezrin/Radixin/Moesin Antibody #3142 (lower). 使用Phospho-Ezrin (Tyr353) (D66D3) Rabbit mAb (上图)或Ezrin/Radixin/Moesin Antibody #3142 (下图),免疫印迹(Western Blot)分析A431细胞中Phospho-Ezrin (Tyr353)和Ezrin/Radixin/Moesin蛋白水平,细胞分为未处理组和hEGF #8916 (100 ng/ml, 5 minutes)处理组。


The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).

ezrin, radixin, 和moesin (ERM)蛋白功能作为细胞质膜和肌动蛋白细胞骨架的连接者,并且该蛋白涉及细胞粘附、胞膜边缘波动(membrane ruffling)和微绒毛形成 (1)。ERM蛋白参与分子内或分子间的氨基和羧基端结构域的相互作用,该蛋白是以非活性的细胞基质单体或二聚体存在 (2)。ERM蛋白在羧基端残基(ezrin蛋白Thr567位点、radixin蛋白Thr564位点、moesin蛋白Thr558 位点) 磷酸化可干扰氨基端和羧基端的关联,这可能在调节ERM蛋白的形成和功能中起着重要作用 (3,4)。ezrin蛋白Thr567位点磷酸化对于细胞骨架的重组和致癌基因诱导的转化可能起着关键角色(5)。基于生长因子刺激,Ezrin蛋白也在酪氨酸残基发生磷酸化。在上皮细胞分化期间,ezrin蛋白Tyr353位点的磷酸化传递一个生存信号(6)。

  1. Tsukita, S. and Yonemura, S. (1999) J Biol Chem 274, 34507-10.
  2. Mangeat, P. et al. (1999) Trends Cell Biol 9, 187-92.
  3. Matsui, T. et al. (1998) J Cell Biol 140, 647-57.
  4. Gautreau, A. et al. (2000) J Cell Biol 150, 193-203.
  5. Tran Quang, C. et al. (2000) EMBO J 19, 4565-76.
  6. Gautreau, A. et al. (1999) Proc Natl Acad Sci U S A 96, 7300-5.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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