Cell Signaling Technology

Product Pathways - NF-kB Signaling

Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb #5483

No. Size Price
5483S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价
5483T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价
5483 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 84 Rabbit IgG
IP 1:50
F 1:50
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey, Xenopus, Bovine, Dog,

Specificity / Sensitivity

Phospho-TBK1 (Ser172) (D52C2) XP® Rabbit mAb detects endogenous levels of TBK1 only when phosphorylated at Ser172.

Phospho-TBK1 (Ser172) (D52C2) XP® Rabbit mAb 只能检测内源的在Ser172位点磷酸化的TBK1蛋白。

Source / Purification

Monoclonal antibody is prepared from animals immunized with a synthetic phosphopeptide corresponding to residues surrounding Ser172 of human TBK1.

此单克隆抗体是通过合成人源对应的TBK1 Ser172位点周围的肽段来免疫动物而获得。

IF-IC

IF-IC

Confocal immunofluorescent analysis of THP-1 cells differentiated with TPA #4174 (80nM, overnight) (left), followed by treatment with LPS (1μg/ml, 1 hour) (center) or LPS with λ phosphatase treatment (right) using Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 Phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析经不同处理的THP-1细胞,TPA #4174 (80nM,过夜) (左图), 经TPA处理后又经LPS (1μg/ml, 1小时)(中间图) 或 LPS 与λ磷酸酶处理(右图),所用抗体为Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb (绿色)。肌动蛋白丝是用DY-554 Phalloidin 标记(红色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml, 1 hour), with or without phosphatase treatment using Phospho-TBK1 (Ser172) (D52C2) XP® Rabbit mAb (upper), or total TBK1/NAK (D1B4) Rabbit mAb #3504 (lower).Western免疫印迹分析THP-1细胞的细胞抽提液,THP-1细胞经过 TPA #4174 (80 nM, 过夜)分化然后用在磷酸酶存在或不存在下用LPS (1 μg/ml, 1小时)处理 所用抗体为Phospho-TBK1 (Ser172) (D52C2) XP® Rabbit mAb (上图) 或 total TBK1/NAK (D1B4) Rabbit mAb #3504 (下图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml), up to 24h, using Phospho-TBK1 (Ser172) (D52C2) XP® Rabbit mAb (upper), or total TBK1/NAK (D1B4) Rabbit mAb #3504 (lower).Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml), up to 24h, using Phospho-TBK1 (Ser172) (D52C2) XP® Rabbit mAb (upper), or total TBK1/NAK (D1B4) Rabbit mAb #3504 (lower).Western免疫印迹分析THP-1细胞的细胞抽提液,THP-1细胞经过 TPA #4174 (80 nM, 过夜)分化然后用LPS (1 μg/ml)处理多达24小时,所用抗体为Phospho-TBK1 (Ser172) (D52C2) XP® Rabbit mAb (上图)或total TBK1/NAK (D1B4) Rabbit mAb #3504 (下图)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of THP-1 cells differentiated with TPA #9905, untreated (blue) or LPS-treated (green), using Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb.流式细胞仪分析经过TPA #9905处理的THP-1细胞,未经其他处理的 (蓝色) 或 LPS处理的(绿色), 所用抗体为Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb。

Background

TBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct phosphorylation (1). TBK1 was identified through association with the TRAF binding protein, TANK, and found to function upstream of NIK and IKK in the activation of NF-κB (2). TBK1/NAK induces IκB degradation and NF-κB activity through IKKβ. NAK may mediate IKK and NF-κB activation in response to growth factors that stimulate PKCε activity (1). TBK1 plays a pivotal role in the activation of IRF3 in the innate immune response (3).

TBK1 (TANK-结合激酶1)/NAK (NF-κB 激活激酶)是IκB激酶(IKK)的激活激酶,能够通过直接磷酸化激活IKK(1)。 TBK1的鉴定是通过与之相关的TRAF结合蛋白——TANK, 并发现在NF-κB的激活中在NIK和IKK 的上游起作用(2)。TBK1/NAK 诱导IκB 的降解和通过IKKβ而激活 NF-κB。生长激素刺激PKCε的活性,NAK 可能是对此作出应答并介导IKK 和 激活NF-κB(1)。TBK1在天然免疫激活IRF3的过程中发挥了重要的作用(3)。

TBK1 is phosphorylated at Ser172 within its activation loop, which is necessary of its kinase activity including the downstream phosphorylation of IRF3 (4,5).

TBK1在活性环内Ser172位点的磷酸化对其激酶的活性是必需的,包括下游的IRF3的磷酸化(4,5)。

  1. Tojima, Y. et al. (2000) Nature 404, 778-82.
  2. Pomerantz, J.L. and Baltimore, D. (1999) EMBO J 18, 6694-704.
  3. Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.
  4. Kishore, N. et al. (2002) J Biol Chem 277, 13840-7.
  5. McCoy, C.E. et al. (2008) J Biol Chem 283, 14277-85.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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