Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Src (Ser17) Antibody #5473

No. Size Price
5473S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5473 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Rat,Monkey, Endogenous 60 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Src (Ser17) Antibody recognizes endogenous levels of Src protein only when phosphorylated at Ser17.

Phospho-Src (Ser17) Antibody兔多抗能够识别内源性Ser17位点磷酸化的Src蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser17 of human Src protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的与人源Src蛋白Ser17位点周围序列相一致的肽段免疫动物而产生的。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 and MCF7 cells, untreated (-) or treated with λ phosphatase (+), using Phospho-Src (Ser17) Antibody or Src (32G5) Rabbit mAb #2123.

使用Phospho-Src (Ser17) Antibody兔多抗和Src (32G5) Rabbit mAb兔单抗 #2123,对未处理(-)和λ磷酸酶处理(+)的C2C12、MCF7细胞提取物进行western blot检测。

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Src家族蛋白酪氨酸激酶包括Src, Lyn, Fyn, Yes, Lck, Blk, Hck, 对调节真核细胞的生长和分化很重要(1)。Src的活性是通过两个酪氨酸位点的磷酸化来调控的,但是这两者的调控效应相反。激酶结构域的活化环中的Tyr416位点的磷酸化,能够上调酶活性;而蛋白羧基端尾部Tyr527位点被Csk磷酸化后,则会使酶的活性降低(2)。

Protein kinase A (PKA)-dependent phosphorylation of Src at Ser17 is thought to influence multiple signaling networks (3-5).

蛋白激酶A(PKA)依赖的Src蛋白Ser17位点的磷酸化,被认为能影响多种信号通路网络(3-5)。

  1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
  2. Hunter, T. (1987) Cell 49, 1-4.
  3. Schmitt, J.M. and Stork, P.J. (2002) Mol Cell 9, 85-94.
  4. Abrahamsen, H. et al. (2003) J Biol Chem 278, 17170-7.
  5. Obara, Y. et al. (2004) J Cell Sci 117, 6085-94.
  6. Hsu, P.P. et al. (2011) Science 332, 1317-22.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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