Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) #5465

4G10   anti-phosphotyrosine   general   motif   phospho-tyrosine   pY100   tyrosine   tyrosine kinase assay  

No. Size Price
5465S 200 µl ( 40 western blots ) ¥3,986.00 现货查询 购买询价
5465 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human,Mouse,Rat,All Species Expected, Endogenous Mouse IgG1
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) is a high affinity antibody. ELISAs against a wide variety of phosphopeptides indicate that P-Tyr-100 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 2D gel western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-100 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-100 does not cross-react with peptides containing phospho-Ser or phospho-Thr. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate)HRP偶联鼠单抗是一个高亲和性的抗体。ELISA中对大量磷酸化肽段的实验显示P-Tyr-100结合磷酸化酪氨酸,不受周围氨基酸序列影响。二维凝胶Western Blot分析过钒酸盐处理处理的细胞抽提物也显示P-Tyr-100与大量酪氨酸磷酸化的蛋白质发生相互作用。P-Tyr-100不与其它包含磷酸化丝氨酸和磷酸化苏氨酸的肽段发生相互作用。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Monoclonal antibody is produced by immunizing animals with phospho-tyrosine containing peptides.

该单克隆抗体用包含磷酸化酪氨酸的肽段免疫动物制备。

Description

This Cell Signaling Technology® antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411.

CST公司生产的抗体与辣根过氧化物酶(HRP)的碳水化合物基团通过其氨基基团相结合。抗体预计可以和未结合HRP的Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411一样具有相同的交叉反应活性。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and 3T3/NIH cells, untreated and treated with sodium vanadate (1 mM for 30 minutes), using Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate).

对NIH/3T3细胞系抽提物,未处理或钒酸钠(1mM,30分钟)处理,使用Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate)进行Western blot分析。

ELISA-Peptide

ELISA-Peptide

Signal-to-noise (S/N) values of phosphopeptide versus nonphosphopeptide signals using LumiGLO® and Peroxide Reagents (#7003).

使用LumiGLO® and Peroxide Reagents #7003的磷酸化肽段和非磷酸化肽段的信噪比。

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

赖氨酸磷酸化在细胞信号转导中扮演重要角色(1)。在肿瘤中,上调酪氨酸激酶活性可以通过产生不适当的增生和存活信号而导致恶性肿瘤和肿瘤形成(2)。具有磷酸化酪氨酸特异性的抗体在这些研究中很有价值(3,4)。Cell Signaling Technology开发的磷酸化酪氨酸单克隆抗体作为非常新的高灵敏度研究工具,在酪氨酸磷酸化和高通量药物发现中监测酪氨酸激酶活性研究中的使用逐渐增加。

  1. Schlessinger, J. (2000) Cell 103, 211-25.
  2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
  3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
  4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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