Cell Signaling Technology

Product Pathways - Phosphatases

Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb #5431

No. Size Price
5431S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5431T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
5431 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse,Rat, Endogenous 72 Rabbit IgG
IP 1:200
F 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry,

Homology

Species predicted to react based on 100% sequence homology: Human,

Specificity / Sensitivity

Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb detects endogenous level of SHP2 only when phosphorylated at Tyr580.

Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb兔单抗能检测内源性Tyr580位点磷酸化的SHP-2蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr580 of human SHP2 protein.

该单克隆抗体是采用合成的与人源SHIP2蛋白Tyr580位点周围残基相一致的磷酸化肽段免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from C6 cells, serum-starved overnight and treated with PDGF (#9909, 50 ng/ml for 5 minutes), using Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb (upper) or total SHP-2 Antibody #3752 (lower).Western blot方法检测细胞提取物:PDGF((#9909, 50 ng/ml 5 min)处理的无血清培养过夜C6细胞。使用的抗体是Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb (上图)和 total SHP-2 Antibody #3752 (下图)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or PDGF-treated (green), using Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb.Flow cytometric方法检测细胞提取物:未处理的(蓝色)和λ磷酸酶PDGF处理的(绿色)NIH/3T3细胞,使用的抗体是Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb。

Background

SHP-2 (PTPN11) is a ubiquitously expressed, nonreceptor protein tyrosine phosphatase (PTP). It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens, and extracellular matrices in the control of cell growth, differentiation, migration, and death (1). Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2). In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites (3). SHP-2 becomes phosphorylated at Tyr542 and Tyr580 in its carboxy-terminus in response to growth factor receptor activation (4). These phosphorylation events are thought to relieve basal inhibition and stimulate SHP-2 tyrosine phosphatase activity (5). Mutations in the corresponding gene result in a pair of clinically similar disorders (Noonan syndrome and LEOPARD syndrome) that may result from abnormal MAPK regulation (6).

SHP-2(PTPN11)是一种广泛表达的非受体酪氨酸磷酸酶(PTP)。它参与了生长因子、细胞因子、激素、抗原以及胞外基质的受体下游的信号转导,这些信号转导过程控制着细胞生长、分化、迁移和死亡(1)。SHP-2的激活以及它与Gab1的结合,对于一些生长因子受体和细胞因子下游的Erk的持续激活是非常重要的(2)。除了在Gab1介导的Erk激活中有重要作用,SHP-2还会通过对Gab1的p85结合位点的脱磷酸化作用,减弱EGF依赖的PI3K激活(3)。在生长因子受体激活的刺激下,SHP-2蛋白羧基端的Tyr542和Tyr580位点被激活(4)。这些磷酸化事件被认为会解除基底的抑制作用,并刺激SHP-2的酪氨酸磷酸酶活性(5)。相应基因的突变会导致两种临床症状相似的紊乱(努南综合症和豹斑综合征),这可能是由于MAPK的调节异常引起的(6)。

  1. Qu, C.K. (2000) Cell Res 10, 279-88.
  2. Maroun, C.R. et al. (2000) Mol Cell Biol 20, 8513-25.
  3. Zhang, S.Q. et al. (2002) Mol Cell Biol 22, 4062-72.
  4. Bennett, A.M. et al. (1994) Proc Natl Acad Sci USA 91, 7335-9.
  5. Lu, W. et al. (2001) Mol Cell 8, 759-69.
  6. Edouard, T. et al. (2007) Cell Mol Life Sci 64, 1585-90.

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