Cell Signaling Technology

Product Pathways - Phosphatases

Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb #5431

No. Size Price
5431S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5431T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
5431 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse,Rat, Endogenous 72 Rabbit IgG
IP 1:200
F 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry,


Species predicted to react based on 100% sequence homology: Human,

Specificity / Sensitivity

Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb detects endogenous level of SHP2 only when phosphorylated at Tyr580.

Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb兔单抗能检测内源性Tyr580位点磷酸化的SHP-2蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr580 of human SHP2 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from C6 cells, serum-starved overnight and treated with PDGF (#9909, 50 ng/ml for 5 minutes), using Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb (upper) or total SHP-2 Antibody #3752 (lower).Western blot方法检测细胞提取物:PDGF((#9909, 50 ng/ml 5 min)处理的无血清培养过夜C6细胞。使用的抗体是Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb (上图)和 total SHP-2 Antibody #3752 (下图)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or PDGF-treated (green), using Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb.Flow cytometric方法检测细胞提取物:未处理的(蓝色)和λ磷酸酶PDGF处理的(绿色)NIH/3T3细胞,使用的抗体是Phospho-SHP-2 (Tyr580) (D66F10) Rabbit mAb。


SHP-2 (PTPN11) is a ubiquitously expressed, nonreceptor protein tyrosine phosphatase (PTP). It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens, and extracellular matrices in the control of cell growth, differentiation, migration, and death (1). Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2). In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites (3). SHP-2 becomes phosphorylated at Tyr542 and Tyr580 in its carboxy-terminus in response to growth factor receptor activation (4). These phosphorylation events are thought to relieve basal inhibition and stimulate SHP-2 tyrosine phosphatase activity (5). Mutations in the corresponding gene result in a pair of clinically similar disorders (Noonan syndrome and LEOPARD syndrome) that may result from abnormal MAPK regulation (6).


  1. Qu, C.K. (2000) Cell Res 10, 279-88.
  2. Maroun, C.R. et al. (2000) Mol Cell Biol 20, 8513-25.
  3. Zhang, S.Q. et al. (2002) Mol Cell Biol 22, 4062-72.
  4. Bennett, A.M. et al. (1994) Proc Natl Acad Sci USA 91, 7335-9.
  5. Lu, W. et al. (2001) Mol Cell 8, 759-69.
  6. Edouard, T. et al. (2007) Cell Mol Life Sci 64, 1585-90.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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