Cell Signaling Technology

Product Pathways - Development

GCNF Antibody #5417

No. Size Price
5417S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
5417 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 50 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

GCNF Antibody detects endogenous levels of total GCNF protein.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic polypeptide corresponding to residues surrounding Ala349 of human GCNF. Antibodies are purified by Protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using GCNF Antibody.用GCNF在western blot实验中检测多种细胞提取物。


GCNF (Germ Cell Nuclear Factor), also known as NR6A1 (Nuclear Receptor Subfamily 6 Group A member), is an orphan member of the nuclear receptor gene superfamily (1). It has been shown to be expressed in the nervous system during development and during specific stages in maturing germ cells of the ovary and testis in the adult, and has probable roles in gametogenesis, neurogenesis, and normal embryonic development during gastrulation (1,2). Inactivation of GCNF in mouse results in abnormal posterior development, impaired midbrain development, insufficient closure of the neural tube, and eventual embryonic death (3). GCNF has been shown to be a repressor of OCT-4 and of the protamine genes (4,5) and plays a critical role in the control of gene expression during embryogenesis and spermatogenesis (2,6).


  1. Lan, Z.J. et al. (2009) Biol Reprod 80, 905-12.
  2. Lei, W. et al. (1997) J Mol Endocrinol 18, 167-76.
  3. Chung, A.C. et al. (2001) Mol Cell Biol 21, 663-77.
  4. Akamatsu, W. et al. (2009) J Neurosci 29, 2113-24.
  5. Hummelke, G.C. and Cooney, A.J. (2004) Mol Reprod Dev 68, 394-407.
  6. Lan, Z.J. et al. (2002) J Biol Chem 277, 50660-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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