Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human His6BAFF/TNFSF13B (hHis6 BAFF) #5413

B lymphocyte stimulator   B-cell-activating factor   BAF   BAF complex   BAFF complex   BLyS   Dendritic cell-derived TNF-like molecule   his tag   TALL-1   TNF- and APOL-related leukocyte expressed ligand 1   Tumor necrosis factor ligand superfamily member 13B  

No. Size Price
5413LC 50 µg ( With Carrier ) ¥7,768.00 现货查询 购买询价
5413LF 50 µg ( Carrier Free ) ¥7,768.00 现货查询 购买询价
5413SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5413SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5413 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human His6BAFF (hHis6BAFF) Ala134-Leu285 (Accession #NP_006564) was expressed in human 293 cells at Cell Signaling Technology.

CST公司生产的人重组蛋白His6BAFF (hHis6BAFF) Ala134-Leu285 (Accession #NP_006564)是从人的293细胞表达而来。

Molecular Characterization

Recombinant N-terminally His6-tagged hBAFF has a calculated MW of 18,066. DTT-reduced and non-reduced protein migrate as 21 kDa polypeptides. The expected amino terminus of recombinant hHis6BAFF was verified by amino acid sequencing.

重组的hBAFF蛋白在N-末端有His6标签,蛋白分子量据推算为18,066Da。DTT-还原的蛋白和未还原的蛋白作为21kDa的蛋白而迁移。重组hHis6BAFF蛋白的N-末端序列通过测序得到。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hHis6BAFF. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) 重组hHis6BAFF通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant hHis6BAFF was determined in a cell proliferation assay using mouse splenic B cells. The ED50 of each lot is between 0.5-2 ng/ml.

hHis6BAFF的生物活性是通过诱导小鼠脾细胞增值能力的实验来确定的。每个批次的ED50在0.5-2 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hHis6BAFF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hHis6BAFF and staining overnight with Coomassie Blue.重组 hHis6BAFF 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组 hHis6BAFF 蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The proliferation of mouse splenic B cells treated with increasing concentrations of hHis6BAFF in the presence of 10 μg/ml of goat anti-mouse IgM μ chain was assessed. After 72 hour treatment with hHis6BAFF, cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.在10 μg/ml羊抗鼠IgM μ 链存在下用递增浓度的hHis6BAFF处理小鼠脾B细胞并观察其增值的实验。用hHis6BAFF培养细胞72小时后与四唑盐孵育并测定 OD450-OD650 数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse splenic B cells, untreated or treated with hHis6BAFF for 24 hours, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).Western免疫印迹。用Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370(上图)和p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (下图) 研究未经处理的和经hHis6BAFF处理24小时的小鼠脾细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1 μg hHis6BAFF.

每微克hHis6BAFF内毒素含量小于0.01 ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 10 mM DTT and 20 μg BSA per 1 μg hHis6BAFF. Cystines are not required for bioactivity. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 10 mM DTT. Cystines are not required for bioactivity.

有载体: 每微克hHis6BAFF蛋白溶解在包含10 mM DTT和20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干,胱氨酸不是生物活性所需要的。

无载体:每微克hHis6BAFF蛋白溶解在包含10 mM DTT的PBS(pH 7.2)溶液,并通过 0.22 μm 滤膜冻干,胱氨酸不是生物活性所需要的。

Background

BAFF, a member of the TNF superfamily of proteins, is a homotrimeric transmembrane protein, which is cleaved to produce a soluble cytokine (1). BAFF may also further oligomerize into 60-mer structures (1). BAFF is expressed by neutrophils, macrophages, dendritic cells, activated T cells, and epithelial cells (1,2). BAFF plays a key role in B cell development, survival, and activation (1,3,4). BAFF binds to three distinct receptors, BAFF-R, TACI, and BCMA (1). These receptors are differentially expressed during B cell development and among B cell subsets (1,2,4). While BAFF-R and BCMA bind to the homotrimeric form of BAFF, TACI only binds to membrane-bound or higher order BAFF structures (1). The BAFF/ BAFF-R interaction activates both canonical and non-canonical NF-κB pathways, PI3K/Akt, and mTor signaling (2,4). Activation of the noncanonical NF-κB pathway via BAFF-R is negatively regulated by TRAF3 (5). Elevated levels of BAFF may exacerbate many autoimmune disorders, making it an attractive therapeutic target (2).

BAFF, TNF超家族的蛋白成员,是一个同源三聚体跨膜蛋白,经过剪切后可以形成可溶性的细胞因子(1)。BAFF 也能进一步的聚合形成60聚体结构(1)。BAFF在中性粒细胞、巨噬细胞、树突状细胞、活化的T细胞和上皮细胞中表达(1,2)。BAFF 在B细胞的发育、存活和激活中发挥了主要的作用(1,3,4)。BAFF结合到3个不同的受体上—BAFF-R、TACI 和 BCMA (1)。这些受体在B细胞及其谱系细胞的发育中具有不同的表达形式(1,2,4)。不像BAFF-R 和BCMA只结合到BAFF的三聚体形式上, TACI只能结合到细胞膜上或者BAFF更高级的结构上(1)。BAFF/ BAFF-R 的相互作用激活典型和非典型的NF-κB信号通路, PI3K/Akt 和 mTOR (2,4)。通过BAFF-R激活非典型的NF-κB信号通路受到TRAF3负调控(5)。研究表明BAFF水平的升高可能加剧许多自身免疫性疾病,这使得它成为治疗的理想靶标(2)。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications