Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat6 (D3H4) Rabbit mAb #5397


No. Size Price
5397S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
5397 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 110 Rabbit IgG
IP 1:50
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Monkey, Bovine, Dog, Pig,

Specificity / Sensitivity

Stat6 (D3H4) Rabbit mAb recognizes endogenous levels of total Stat6 protein.

Stat6 (D3H4) Rabbit mAb 能够检测内源Stat6蛋白总体水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly582 of human Stat6 protein.

此单克隆抗体是通过合成人源对应的Stat6 Gly582位点周围的肽段来免疫动物而获得。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Ramos cells starved overnight then treated with IL-4 (100 ng/ml, 30 min) and either 10 μl of Stat6 (D3H4) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human DMD Intron 2 Primers #7710, human MS4A1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。Ramos细胞培养至4 x 106,饥饿过夜并经IL-4 (100 ng/ml)处理30分钟,然后用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用SimpleChIP® Human DMD Intron 2 Primers #7710, human MS4A1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486引物对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Stat6 (D3H4) Rabbit mAb.Western免疫印迹。用Stat6 (D3H4) Rabbit mAb研究各种细胞系的细胞提取液。


Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cells, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).

受到Janus激酶激活后, Stat6 入核并调控细胞素诱导基因的表达。Stat6 通过在Tyr641位点的磷酸化激活,这对IL-4 和 IL-13的应答是必需的(1-4)。另外, 在B细胞系中Stat6被 IFN-α 激活并与 Stat2和 p48形成具有转录活性的复合体(5,6)。蛋白磷酸酶 2A 也参与到 IL-4介导的 Stat6信号通路中 (7)。

  1. Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
  2. Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
  3. Hou, J. et al. (1994) Science 265, 1701-1706.
  4. Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
  5. Takeda, K. et al. (1996) Nature 380, 627-630.
  6. Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
  7. Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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