Product Pathways - Vesicle Trafficking
STAM2 (D8B3G) Rabbit mAb #53674
|53674S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|53674||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
STAM2 (D8B3G) Rabbit mAb recognizes endogenous levels of total STAM2 protein. This antibody does not cross-react with STAM1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human STAM2 protein.
Western blot analysis of extracts from various cell lines using STAM2 (D8B3G) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human STAM1 protein (hSTAM1-Myc/DDK; +) or Myc/DDK-tagged full-length human STAM2 protein (hSTAM2-Myc/DDK; +), using STAM2 (D8B3G) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower).
Signal transducing adaptor molecule 2 (STAM2) is a ubiquitously expressed STAM family adaptor protein and an integral component of the ESCRT-0 complex. Similar to STAM1, STAM2 possesses a single SH3 domain and an immunoreceptor tyrosine-based activation motif (ITAM). Following activation of multiple growth factor and cytokine cell surface receptors, the STAM2 protein undergoes tyrosine phosphorylation and potentiates mitogenic signals driven by these receptors (1,2). Research studies demonstrate that STAM2 is localized to complexes containing Eps15, Hrs, and STAM1 proteins on early endosome membranes. A tandem, amino-terminal VHS (Vps27/Hrs/STAM) domain and UIM (ubiquitin-interacting) motif within STAM2 facilitate STAM2 interaction with ubiquitinated cargo proteins, suggesting that this adaptor participates in the endosomal sorting of ubiquitinated proteins targeted for lysosomal degradation (3-6). Gene targeting studies have revealed an indispensible role for STAM2 in T-cell development (7).
- Endo, K. et al. (2000) FEBS Lett 477, 55-61.
- Pandey, A. et al. (2000) J Biol Chem 275, 38633-9.
- Mizuno, E. et al. (2003) Mol Biol Cell 14, 3675-89.
- Bache, K.G. et al. (2003) J Biol Chem 278, 12513-21.
- Takata, H. et al. (2000) Genes Cells 5, 57-69.
- Mizuno, E. et al. (2004) J Biochem 135, 385-96.
- Yamada, M. et al. (2002) Mol Cell Biol 22, 8648-58.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
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