Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb #5364

ps6  

No. Size Price
5364L 300 µl ( 30 western blots ) ¥9,458.00 现货查询 购买询价
5364S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价
5364T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价
5364 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 32 Rabbit IgG
IHC-P 1:2000
F 1:200
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Chicken, Pig,

Specificity / Sensitivity

Phospho-S6 Ribosomal Protein (Ser235/236) (D68F8) XP® Rabbit mAb detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser240 and Ser244.

Phospho-S6 Ribosomal Protein (Ser235/236) (D68F8) XP® Rabbit mAb兔单抗检测仅在Ser240 和Ser244位点磷酸化的内源性核糖体蛋白S6的水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser240 and Ser244 of human ribosomal protein S6.

通过人工合成人源核糖体蛋白S6的Ser240和Ser244位点周围相应的磷酸化片段去免疫动物从而制备单克隆抗体。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis on SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cell pellets -/+ LY294002) using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb.

使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗,免疫组化分析SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101 (石蜡包埋的LNCaP细胞沉淀物, -/+ LY294002处理)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗,免疫组化分析人类结肠癌组织石蜡切片,其中分别封闭孵育对照多肽(左图)和抗原特异的多肽 (右图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded LNCaP cell pellets, control (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb.

使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗,免疫组化分析LNCaP细胞沉淀的石蜡切片,分为对照组(左图)和rapamycin处理组(右图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb.

使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗,免疫组化分析人类肺癌组织石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Rh30 xenograft, control (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb.

使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗,免疫组化分析Rh30异种移植物的石蜡切片,分为对照组(左图)和rapamycin处理组(右图)。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, insulin-treated (left) and LY294002-treated (#9901, right), using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗(绿色),共聚焦免疫荧光分析HeLa细胞系,分为胰岛素处理组(左图)和LY294002处理组(右图)。DY-554 phalloidin标记微丝蛋白(红色)。蓝色伪彩= DRAQ5® #4084(DNA荧光染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 and NIH/3T3 cells, treated with 100 nM insulin (10 min) or 20% FBS (30 min) as indicated, using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower). 使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb (上图)和S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (下图),免疫印迹(Western blot)分析MCF7和NIH/3T3细胞,如图所示分为100 nM insulin (10 min)处理组和20% FBS(30 min)处理组。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002, wortmannin and U0126 (blue), using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb.

使用Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗,流式细胞术分析Jurkat细胞,细胞分为对照组(绿色)和LY294002、wortmannin和U0126处理组(蓝色)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse colon using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb.

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

生长因子和有丝分裂原有效促进维持细胞生长和增殖的一个方面就是通过上调mRNA翻译(1,2)。生长因子和有丝分裂原能诱导p70 S6激酶的激活和随后S6核糖体蛋白的磷酸化。S6核糖体蛋白的磷酸化与mRNA转录本的翻译增加有关,而这个mRNA转录本是在5端非翻译区含有一个寡嘧啶区(2)。这些特殊的mRNA转录本 (5'TOP) 能够编码涉及细胞周期进程的蛋白质如翻译必须的核糖体蛋白和延伸因子(2,3)。重要的S6核糖体蛋白的磷酸化位点包含数个定位在S6蛋白一个小的羧基末端区域残基(Ser235, Ser236, Ser240, and Ser244) (4,5)。

  1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
  2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
  3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
  4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
  5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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