Cell Signaling Technology

Product Pathways - Neuroscience

CLIC1 (D7D6H) Rabbit mAb #53424

No. Size Price
53424S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
53424 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 22 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

CLIC1 (D7D6H) Rabbit mAb recognizes endogenous levels of total CLIC1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CLIC1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from K-562, MDA-MB-231, and C2C12 cells using CLIC1 (D7D6H) Rabbit mAb.



Immunoprecipitation of CLIC1 from C2C12 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CLIC1 (D7D6H) Rabbit mAb. Western blot analysis was performed using

CLIC1 (D7D6H) Rabbit mAb.



Chloride intracellular channel (CLIC) proteins belong to a family of highly conserved transport proteins found as both soluble and membrane-bound forms (1). Although CLIC proteins have putative, selective chloride ion channel activity, they are structural homologs to members of the glutathione-S-transferase protein superfamily and are likewise regulated by redox status (2). CLIC proteins are distinct from other ion channels in that they are found as both soluble and integral membrane forms, and their form determines their function (3-6). Chloride intracellular channel proteins are ubiquitously expressed in numerous tissue types and are involved in diverse biological functions (1,2).

CLIC1 is a member of the CLIC protein family. It is ubiquitously expressed in many tissues and organs (7). CLIC1 is overexpressed in multiple tumor types and has been implicated in the proliferation, migration, and invasion of these tumors (8-11). In the central nervous system, CLIC1 protein expression is elevated upon amyloid β-peptide treatment in neonatal rat microglia. Inhibition of CLIC1 prevents neuronal apoptosis in neurons co-cultured with amyloid β-peptide treated microglia (12). Further studies indicate that CLIC1 translocates from the cytosol to the plasma membrane of microglia upon exposure to amyloid β-peptide, and contributes to the subsequent neurotoxicity through generation of superoxide anions (13). These discoveries implicate CLIC1 as a possible therapeutic target for Alzheimer’s disease.

  1. Littler, D.R. et al. (2010) FEBS Lett 584, 2093-101.
  2. Oakley, A.J. (2005) Curr Opin Struct Biol 15, 716-23.
  3. Littler, D.R. et al. (2005) FEBS J 272, 4996-5007.
  4. Singh, H. and Ashley, R.H. (2006) Biophys J 90, 1628-38.
  5. Suh, K.S. et al. (2004) J Biol Chem 279, 4632-41.
  6. Fernández-Salas, E. et al. (2002) Mol Cell Biol 22, 3610-20.
  7. Ulmasov, B. et al. (2007) BMC Cell Biol 8, 8.
  8. Wei, X. et al. (2015) J Gastroenterol Hepatol 30, 208-16.
  9. Gurski, L.A. et al. (2015) Mol Cancer Res 13, 273-80.
  10. Ding, Q. et al. (2015) Tumour Biol 36, 193-8.
  11. Tian, Y. et al. (2014) Cancer Biother Radiopharm 29, 339-44.
  12. Novarino, G. et al. (2004) J Neurosci 24, 5322-30.
  13. Milton, R.H. et al. (2008) J Neurosci 28, 11488-99.

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