Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Vascular Endothelial Growth Factor-120 (mVEGF120 ) #5314


No. Size Price
5314LC 50 µg ( With Carrier ) ¥7,768.00 现货查询 购买询价
5314LF 50 µg ( Carrier Free ) ¥7,768.00 现货查询 购买询价
5314SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5314SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5314 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse VEGF120 (mVEGF120) Ala27-Arg146 (Accession #NP_001020428) was expressed in human 293 cells at Cell Signaling Technology.

CST公司生产的鼠源重组蛋白VEGF120 (mVEGF120) Ala27-Arg146 (Accession #NP_001020428)是从人源293细胞表达而来。

Molecular Characterization

Recombinant mVEGF120 contains no "tags" and the nonglycosylated protein has a calculated MW of 14,071. DTT-reduced protein migrates as a 20-26 kDa polypeptide. Lower mobility in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 32-37 kDa protein. The expected amino-terminal APTTE of recombinant hVEGF120 was verified by amino acid sequencing.

重组的mVEGF120蛋白没有标签,未糖基化的蛋白分子量据推算为14,071Da。DTT-还原蛋白由于糖基化的存在而作为一个20-26kDa的蛋白转移,因此在SDS-PAGE中有较低的迁移率。未还原的蛋白由于有半胱氨酸连接形成同源二聚体而作为32-37 kDa 蛋白迁移。重组hVEGF120蛋白的N-末端APTTE的序列通过测序得到。


>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mVEGF120. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) mVEGF120通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。


The bioactivity of recombinant mVEGF120 was determined in a cell proliferation assay using HUVEC. The ED50 of each lot is between 1-5 ng/ml.

重组蛋白mVEGF120的生物活性是通过HUVEC细胞的增殖实验确定的。每个批次的ED50值在1-5 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mVEGF120 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mVEGF120 and staining overnight with Coomassie Blue.重组mVEGF120 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mVEGF120蛋白跑SDS胶并用考马斯亮蓝染色过夜。



The proliferation of HUVEC treated with increasing concentrations of mVEGF120 was assessed. After 72 hour treatment with mVEGF120 cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.在mVEGF120 蛋白浓度递增条件下研究HUVEC细胞增殖实验。用mVEGF120培养细胞72小时后与四唑盐孵育。并测定OD450 - OD650值。

Western Blotting

Western Blotting

Western blot analysis of extracts from HUVEC untreated or treated with mVEGF120 for 15 minutes, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) or Akt1 (C73H10) Rabbit mAb #2938 (lower).Western免疫印迹。用Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (上图) 和Akt1 (C73H10) Rabbit mAb #2938 (下图) 研究未经处理的和经mVEGF120处理15 min的HUVEC细胞的细胞提取液。


Less than 0.01 ng endotoxin/1μg mVEGF120.

内毒素含量:小于0.01 ng /1 μg mVEGF120。


With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mVEGF120. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克mVEGF120蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mVEGF120蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。


VEGF120 , a splice variant of the VEGF-A gene, is the murine counterpart to human VEGF121 (1,2). VEGF120 is produced by endothelial cells, macrophages, T cells, and others. VEGF120 is involved in angiogenesis, vascular permeability, vascular endothelial cell survival, growth, and migration (1). VEGF120 expression is induced by hypoxia, inflammatory cytokines, and through oncogene products in tumors (1,2). VEGF120 binds to VEGFR1 and VEGFR2 receptor tyrosine kinases (1). Binding of VEGF120 to VEGFR1 and VEGFR2 leads to activation of pathways involving PI3K/Akt, p38, and FAK (1). VEGF plays a key role in tumor angiogenesis in many cancers (2).

VEGF120 ,是VEGF-A基因的一种剪切形式,其存在于鼠科中,与人的VEGF121形式及其相似(1,2)。VEGF120能够通过内皮细胞、巨噬细胞、T细胞和其它的一些细胞类型产生。VEGF120参与到了血管再生、血管内皮细胞生存、生长、迁移和血管渗透(1)。VEGF120的表达受到组织缺氧、炎症因子和肿瘤组织产生的致癌基因的诱导(1,2)。结合到VEGFR1和VEGFR2 受体酪氨酸激酶(1)。VEGF121 结合到 VEGFR1和VEGFR2导致了信号通路的激活,如PI3K/AKT, P38 MAPK和 FAK (1)。在很多癌症中,VEGF在肿瘤血管再生中发挥了重要的作用(2)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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