Cell Signaling Technology

Product Pathways - Development

RBPSUH (D10A4) XP® Rabbit mAb #5313

Notch   sc-28713   Suppressor of Hairless  

No. Size Price
5313S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
5313T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
5313 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 61 Rabbit IgG
IHC-P 1:2600
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), ChIP=Chromatin IP,

Specificity / Sensitivity

RBPSUH (D10A4) XP® Rabbit mAb recognizes endogenous levels of total RBPSUH protein.

RBPSUH (D10A4) XP® Rabbit mAb可以识别内源性的总RBPSUH蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln110 of human RBPSUH protein.

单克隆抗体由合成肽段免疫动物产生,该肽段与人RBPSUH蛋白邻近110位谷氨酰胺氨基酸残基序列一致。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RBPSUH (D10A4) XP® Rabbit mAb.

使用RBPSUH (D10A4) XP® Rabbit mAb对多种细胞提取物进行western blot分析。

Chromatin IP

Chromatin IP

CUTLL1 cells were cultured in media with γ-secretase inhibitor (1 μM, 3 d) and then either harvested immediately (left panel) or washed and cultured in fresh media for 3 h (right panel). Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 cells and 10 µl of RBPSUH (D10A4) XP® Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human HES1 promoter primers, SimpleChIP® Human HES4 Promoter Primers #7273, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

培养基中的CUTLL1经过 γ-分泌酶 抑制剂 (1 μM, 3 d)处理,随后立即收集(left panel)或冲洗后在新鲜培养基中孵育3h(right panel)。然后对来自4 x 106细胞的交联染色质进行染色质免疫共沉淀实验,使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003中的10µl RBPSUH (D10A4) XP® Rabbit mAb或2µl Normal Rabbit IgG #2729。富集的DNA使用人HES1启动子引物,impleChIP® Human HES4 启动子引物 #7273,和SimpleChIP® Human α Satellite Repeat 引物 #4486以real-time PCR的方式定量。各样品沉淀得到的DNA量通过与input染色质总量对比获得,每份Input的染色质量设定值为1。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse lymph node using RBPSUH (D10A4) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using RBPSUH (D10A4) XP® Rabbit mAb.

使用RBPSUH (D10A4) XP® Rabbit mAb兔单抗对石蜡包埋的人肺癌组织进行免疫组化染色分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded E18.5 mouse lung, Rbpjk F/+ Shh+/+ (wild type, left) or Rbpjk F/- Shhcre/+ (Rbpjk conditional knock out, right), using RBPSUH (D10A4) XP® Rabbit mAb. Note lack of staining in the bronchial epithelial cells in the conditional knock out tissue (right). Tissue courtesy of Dr. Wellington Cardosa, Boston University School of Medicine.

使用RBPSUH (D10A4) XP® Rabbit mAb兔单抗对石蜡包埋的E18.5 小鼠肺组织, Rbpjk F/+ Shh+/+ (野生型,左) 或Rbpjk F/- Shhcre/+ (Rbpjk 选择性敲除, 右)进行免疫组化染色分析。显示对于选择性敲除的人支气管上皮细胞(右)染色缺失,组织样品由Dr. Wellington Cardosa, Boston University School of Medicine馈赠。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using RBPSUH (D10A4) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

在对照肽段(左)或抗原特异性肽段(右)存在的情况下,使用RBPSUH (D10A4) XP® Rabbit mAb兔单抗对石蜡包埋的人肺癌组织进行免疫组化染色分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using RBPSUH (D10A4) XP® Rabbit mAb.

使用RBPSUH (D10A4) XP® Rabbit mAb兔单抗对石蜡包埋的人结肠癌组织进行免疫组化染色分析。

Background

RBPSUH (Recombining Binding Protein, SUppressor of Hairless), also termed RBP-J or CSL, is the DNA-binding component of the transcription complex regulated by canonical Notch signaling. In the absence of Notch activation, RBPSUH suppresses target gene expression through interactions with a co-repressor complex containing histone deacetylase. Upon activation of Notch receptors, the Notch intracellular domain (NICD) translocates to the nucleus and binds to RBPSUH. This displaces the co-repressor complex and replaces it with a transcription activation complex that includes Mastermind-like (MAML) proteins and histone acetylase p300, leading to transcriptional activation of Notch target genes (1-3). RBPSUH is also the DNA-binding partner for Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2), a protein critical for latent viral transcription and immortalization of EBV-infected B cells (4,5).

RBPSUH(Recombining Binding Protein, SUppressor of Hairless),也称为RBP-J或CSL,是canonical Notch 信号通路调控的转录复合物的DNA结合组分。在缺少Notch激活的情况下,RBPSUH通过与一个含有组蛋白去乙酰基酶的共抑制复合物结合发挥抑制靶基因表达的作用。激活Notch受体后,Notch细胞内结构域(NICD)转位到细胞核,并与RBPSUH结合。此时共抑制复合物会被置换为一个转录激活复合物包括Mastermind-like (MAML)蛋白和组蛋白乙酰化酶p300,引起Notch靶基因的转录激活(1-3)。RBPSUH也是Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2)的DNA结合伴侣,EBNA2是一个对潜伏病毒转录和EBV-感染的B细胞永生化非常重要的蛋白(4,5)。

  1. Ehebauer, M. et al. (2006) Sci STKE 2006, cm7.
  2. Borggrefe, T. and Oswald, F. (2009) Cell Mol Life Sci 66, 1631-46.
  3. Kopan, R. and Ilagan, M.X. (2009) Cell 137, 216-33.
  4. Henkel, T. et al. (1994) Science 265, 92-5.
  5. Strobl, L.J. et al. (1997) Immunobiology 198, 299-306.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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