Cell Signaling Technology

Product Pathways - Phosphatases

Phospho-CTDSPL2 (Ser104) Antibody #5294

No. Size Price
5294S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5294 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 61 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-CTDSPL2 (Ser104) Antibody detects endogenous levels of CTDSPL2 only when phosphorylated at Ser104.

Phospho-CTDSPL2 (Ser104) Antibody检测仅在Ser104位点磷酸化的内源性CTDSPL2总蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser104 of human CTDSPL2 protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from NCI-H1703 cells, untreated or EGF-treated, and HeLa cells, untreated or λ-phosphatase-treated, using Phospho-CTDSPL2 (Ser104) Antibody (upper) and a total CTDSPL2 antibody (lower).

使用Phospho-CTDSPL2 (Ser104) Antibody (上图)和总的CTDSPL2 antibody (下图),免疫印迹(Western blot)分析未处理或EGF处理的NCI-H1703和HeLa细胞中Phospho-CTDSPL2 (Ser104)和CTDSPL2的蛋白水平。


CTD small phosphatase-like protein 2 (CTDSPL2, HSPC129) is a putative RNA-polymerase II carboxy-terminal domain (CTD) phosphatase (1) that belongs to a small subfamily of CTD phosphatases (2). The CTD of RNA polymerase II contains multiple Y-S-P-T-S-P-S repeats that are phosphorylated during the transcription cycle (3,4). In general, CTD phosphatases regulate the reversible CTD phosphorylation state of RNA-polymerase II at several stages of RNA synthesis and during post-transcriptional modification (4-6). CTDSPL2 has several structural and functional similarities to other CTD phosphatases, including FCP1, SCP1, DULLARD, and UBLCP1 (1,2).

Phosphorylation of CTDSPL2 at Ser104 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery (7). The site was independently found in select carcinoma cell lines and in tumors (8).

CTD small phosphatase-like protein 2 (CTDSPL2, HSPC129)是一个假设RNA-polymerase II carboxy-terminal domain (CTD)磷酸酶(1),这属于CTD磷酸酶的一个小亚家族(2)。RNA polymerase II的CTD包含多重的Y-S-P-T-S-P-S重复序列,该序列是在转录循环期间被磷酸化(3,4)。一般而言,在RNA合成的数个步骤和在转录后修饰期间,CTD磷酸酶调节RNA-polymerase II的可逆CTD磷酸化作用(4-6)。CTDSPL2蛋白有数个结构性和功能性类似与其它CTD磷酸酶,包括FCP1、SCP1、DULLARD和UBLCP1 (1,2)。

Cell Signaling Technology (CST)使用PhosphoScan® 和LC-MS/MS平台发现了CTDSPL2蛋白在Ser104位点的磷酸化(7)。在选择癌细胞系和肿瘤中也发现了该位点(8)。

  1. Qian, H. et al. (2007) Mol Cell Biochem 303, 183-8.
  2. Kim, Y. et al. (2007) Proc Natl Acad Sci USA 104, 6596-601.
  3. Corden, J.L. et al. (1985) Proc Natl Acad Sci USA 82, 7934-8.
  4. Ahn, S.H. et al. (2004) Mol Cell 13, 67-76.
  5. Dahmus, M.E. (1996) J Biol Chem 271, 19009-12.
  6. Goodrich, J.A. and Tjian, R. (1994) Cell 77, 145-56.
  7. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
  8. Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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