Cell Signaling Technology

Product Pathways - Protein Stability

UBA2 Antibody #5293

ubiquitin  

No. Size Price
5293S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
5293 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 90 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

UBA2抗体识别内源性的UBA2总蛋白。

Source / Purification

UBA2单克隆抗体是通过合成与人UBA2蛋白Leu490邻近的氨基酸残基序列一致的肽段,免疫动物获得,抗体经Protein A和肽亲和层析纯化。 Western blot analysis of extracts from various cell lines using UBA2 Antibody. 使用UBA2抗体对不同细胞裂解物进行免疫印迹分析的结果。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using UBA2 Antibody.

Background

SUMO修饰结合于目标蛋白质的过程是类似于泛素化修饰的事件(1)。SUMO是通过由E1,E2和E3酶(2)组成的SUMO修饰系统协同作用而连接到靶向蛋白。典型的SUMOE1激活酶是由SAE1(AOS1)和UBA2(SAE2)亚基组成的异源二聚体。成熟SUMO是由激活酶E1通过依赖于ATP的反应,生成腺苷酸化SUMO(SUMO与UBA2的Cys173之间形成硫酯键而形成的一种高能中间体)(3,4)。SUMO然后从UBA2转移到SUMO的E2结合酶UBC9(5)。最近的证据表明,UBA2氧化还原调控作为一个生理机制,调节sumo化靶蛋白的细胞水平(6)。

  1. Geiss-Friedlander, R. and Melchior, F. (2007) Nat Rev Mol Cell Biol 8, 947-56.
  2. Tatham, M.H. et al. (2003) Biochemistry 42, 9959-69.
  3. Desterro, J.M. et al. (1999) J Biol Chem 274, 10618-24.
  4. Gong, L. et al. (1999) FEBS Lett 448, 185-9.
  5. Desterro, J.M. et al. (1997) FEBS Lett 417, 297-300.
  6. Bossis, G. and Melchior, F. (2006) Mol Cell 21, 349-57.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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