Cell Signaling Technology

Product Pathways - Apoptosis

Phospho-Bad (Ser136) (185D10) Rabbit mAb #5286

No. Size Price
5286S 200 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5286T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
5286 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:500 Mouse,Monkey, Transfected Only 23 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Human, Rat,

Specificity / Sensitivity

Phospho-Bad (Ser136) (185D10) Rabbit mAb detects transfected levels of Bad protein only when phosphorylated at Ser136.

Phospho-Bad (Ser136) (185D10) Rabbit mAb 兔单抗只能检测内源的在Ser136位点磷酸化的Bad。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser136 of mouse Bad.

该单克隆抗体是采用合成的小鼠源Bad Ser136位点周围残基相对应的多肽来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from control 293, GST-Bad transfected 293 and COS cells, untreated or TPA-treated, using Phospho-Bad (Ser136) (185D10) Rabbit mAb.Western blot免疫印迹分析对照293细胞,GST-Bad转染的293细胞和COS细胞的细胞提取液,未经过处理和经过TPA处理的,所用抗体为Phospho-Bad (Ser136) (185D10) Rabbit mAb 兔单抗。


Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).

Bad是Bcl-2促凋亡家族中的成员,它通过转移 Bax 与 Bcl-2 和 Bcl-xL 结合而促进细胞死亡(1,2)。存活因子如IL-3, 能够通过激活细胞内信号通路,导致Bad在Ser112和Ser136位点发生磷酸化,进而抑制凋亡活性(2)。在这些位点的磷酸化促进Bad与 14-3-3 蛋白的结合,从而阻止了Bad与Bcl-2和Bcl-xl的互作(2)。Akt在Ser136位点磷酸化Bad,而促进细胞的存活(3,4)。Bad在体内和体外都能够被p90RSK(5,6)和线粒体锚定PKA磷酸化Ser112位点(7)。PKA对BH3结构域Ser155位点的磷酸化,在阻止Bad和Bcl-xL的二聚化过程中发挥了重要的作用(8-10)。

  1. Yang, E. et al. (1995) Cell 80, 285-291.
  2. Zha, J. et al. (1996) Cell 87, 619-628.
  3. Datta, S.R. et al. (1997) Cell 91, 231-241.
  4. Peso, L. et al. (1997) Science 278, 687-689.
  5. Bonni, A. et al. (1999) Science 286, 1358-1362.
  6. Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859-34867.
  7. Harada, H. et al. (1999) Mol. Cell 3, 413-422.
  8. Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869.
  9. Lizcano, J. et al. (2000) Biochem. J. 349, 547-557.
  10. Datta, S. et al. (2000) Mol. Cell 6, 41-51.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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