Cell Signaling Technology

Product Pathways - Apoptosis

Phospho-Bad (Ser112) (40A9) Rabbit mAb #5284

bcl   bcl2  

No. Size Price
5284L 300 µl ( 30 western blots ) ¥9,325.00 现货查询 购买询价
5284S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5284T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
5284 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 23 Rabbit IgG
IHC-P 1:50
F 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry,

Specificity / Sensitivity

Phospho-Bad (Ser112) (40A9) Rabbit mAb detects endogenous levels of Bad only when phosphorylated at Ser112. The Ser112 nomenclature is based upon the mouse sequence. The analogous phosphorylation site is Ser75 in human and Ser113 in rat. This antibody does not detect Bad phosphorylated at other sites, nor does it detect related family members.

Phospho-Bad (Ser112) (40A9) Rabbit mAb 兔单抗只能检测内源的在Ser112位点磷酸化的Bad。Ser112的系统命名法是建立在小鼠源蛋白序列的基础上。其人和大鼠的同工蛋白分别是 Ser75和Ser113。此抗体不与其它位点磷酸化的Bad反应,也不能识别相关的家族成员。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser112 of mouse Bad.

该单克隆抗体是通过合成对应小鼠源Bad Ser112位点周围残基的多肽来免疫动物而获得。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin embedded COS cells untreated (left) or TPA-treated (right), showing induced cytoplasmic staining using Phospho-Bad (Ser112) (40A9) Rabbit mAb.

免疫组化分析石蜡包埋的未经处理(左图)或者TPA处理 (右图)的COS细胞,图示为诱导的细胞质染色,所用抗体为Phospho-Bad (Ser112) (40A9) Rabbit mAb 兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Non-Hodgkin's lymphoma, using Phospho-Bad (Ser112) (40A9) Rabbit mAb.

免疫组化分析石蜡包埋的非霍奇金淋巴瘤,所用抗体为Phospho-Bad (Ser112) (40A9) Rabbit mAb 兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Phospho-Bad (Ser 112) (40A9) Rabbit Monoclonal Antibody preincubated with control peptide (left) or Phospho-Bad (Ser 112) Blocking Peptide (IHC Specific) #1026 (right).

免疫组化分析石蜡包埋的人前列腺癌组织,经过对照多肽(左图) 或者Phospho-Bad (Ser 112)Blocking Peptide (IHC Specific) #1026 预孵育(右图)后, 再用Phospho-Bad (Ser112) (40A9) Rabbit mAb 兔单抗检测。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or lambda phosphatase treated (right), using Phospho-Bad (Ser 112) (40A9) Rabbit mAb.

免疫组化分析石蜡包埋的人乳腺癌组织,未经处理(左侧) 或者 lambda 磷酸酶处理(右图), 所用抗体为Phospho-Bad (Ser112) (40A9) Rabbit mAb 兔单抗。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of COS cells, untreated (blue) or TPA/Calyculin A treated (green), using Phospho-Bad (Ser112) (40A9) Rabbit mAb.

流式细胞仪分析未经处理(蓝色) 或者经过 TPA/Calyculin A 处理(绿色)的COS 细胞的细胞提取液, 所用抗体为Phospho-Bad (Ser112) (40A9) Rabbit mAb 兔单抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untreated or TPA-treated, using Phospho-Bad (Ser112) (40A9) Rabbit mAb (upper) or Bad Antibody #9292 (lower).

Western blot 免疫印迹分析未经处理或经过TPA处理的COS 细胞的细胞提取液,用Phospho-Bad (Ser112) (40A9) Rabbit mAb 兔单抗 (上图) 或者 Bad Antibody #9292 (下图) 。


Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).

Bad是Bcl-2促凋亡家族中的成员,它通过转移 Bax 与 Bcl-2 和 Bcl-xL 结合而促进细胞死亡(1,2)。存活因子如IL-3, 能够通过激活细胞内信号通路,导致Bad在Ser112和Ser136位点发生磷酸化,进而抑制凋亡活性(2)。在这些位点的磷酸化促进Bad与 14-3-3 蛋白的结合,从而阻止了Bad与Bcl-2和Bcl-xl的互作(2)。Akt在Ser136位点磷酸化Bad,而促进细胞的存活(3,4)。Bad在体内和体外都能够被p90RSK(5,6)和线粒体锚定PKA磷酸化Ser112位点(7)。PKA对BH3结构域Ser155位点的磷酸化,在阻止Bad和Bcl-xL的二聚化过程中发挥了重要的作用(8-10)。

  1. Yang, E. et al. (1995) Cell 80, 285-291.
  2. Zha, J. et al. (1996) Cell 87, 619-628.
  3. Datta, S.R. et al. (1997) Cell 91, 231-241.
  4. Peso, L. et al. (1997) Science 278, 687-689.
  5. Bonni, A. et al. (1999) Science 286, 1358-1362.
  6. Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859-34867.
  7. Harada, H. et al. (1999) Mol. Cell 3, 413-422.
  8. Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869.
  9. Lizcano, J. et al. (2000) Biochem. J. 349, 547-557.
  10. Datta, S. et al. (2000) Mol. Cell 6, 41-51.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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