Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-TCTP (Ser46) Antibody #5251

fortillin   histamine-releasing factor   HRF   p23   TPT1   translationally-controlled tumor protein  

No. Size Price
5251S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5251 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 23 Rabbit
IHC-P 1:100
F 1:100
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-TCTP (Ser46) Antibody recognizes endogenous levels TCTP protein only when phosphorylated at Ser46. In some cell types the antibody may recognize an 80-100 kDa protein of unknown origin.

Phospho-TCTP (Ser46) Antibody检测仅在Ser46位点磷酸化的内源性TCTP蛋白。在一些细胞类型中,该抗体可能识别一个80-100 kDa大小的未知来源的蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser46 of human TCTP protein. Antibodies are purified by protein A and peptide affinity chromatography.

通过人工合成人源TCTP蛋白Ser46位点周围相应的磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from U-2 OS cells, untreated or synchronized in mitosis by thymidine block followed by release into nocodazole, using Phospho-TCTP (Ser46) Antibody.

使用Phospho-TCTP (Ser46) Antibody,免疫印迹(Western Blot)分析U-2 OS细胞中Phospho-TCTP蛋白水平。细胞分为untreated或Mitotic synchrony通过使用thymidine封闭随后nocodazole处理。

IF-IC

IF-IC

Confocal immunofluorescent analysis of U-2 OS cells using Phospho-TCTP1 (Ser46) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用Phospho-TCTP1 (Ser46) Antibody (绿色)标记,共聚焦免疫荧光分析U-2 OS细胞。DY-554 phalloidin标记微丝蛋白(红色)。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Phospho-TCTP (Ser46) Antibody compared to propidium iodide (DNA content).

与propidium iodide (DNA含量)比较,使用Phospho-TCTP (Ser46) Antibody,流式细胞仪分析Jurkat细胞。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HT-29 cell pellets, control (left) or nocodazole-treated (right), using Phospho-TCTP (Ser46) Antibody.

使用Phospho-TCTP (Ser46) Antibody,免疫组化分析HT-29细胞石蜡切片,组织分为control (左图)或nocodazole-treated (右图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or lambda-phosphatase-treated (right), using Phospho-TCTP (Ser46) Antibody.

使用Phospho-TCTP (Ser46) Antibody,免疫组化分析人源乳腺癌组织石蜡切片,组织分为control (左图)或lambda-phosphatase-treated (右图)。

Background

Translationally controlled tumor protein (TCTP/p23/HRF) is a ubiquitously expressed and highly conserved protein involved in various cellular processes, including a role as a histamine releasing factor in chronic allergic disease (1). TCTP binds tubulin in a cell cycle dependent manner and is associated with the mitotic spindle (2). In addition, TCTP interacts with the actin cytoskeleton to regulate cell shape (3). In mitosis, TCTP is phosphorylated by PLK at Ser46, decreasing microtubule stability (4,5). TCTP interacts with the small GTPase Rheb, possibly acting as a GEF and thus activating the TORC1 pathway, controlling cell growth and proliferation (6,7). TCTP has also been shown to be involved in apoptosis and cell stress (8-11). In cultured cells, reduction of TCTP expression can cause tumor reversion (12).

Translationally controlled tumor protein (TCTP/p23/HRF)是一个广泛表达的,并且高度保守的蛋白,该蛋白涉及不同细胞内进程,包括作为一个在慢性变态反应性疾病中组胺释放因子的角色(1)。TCTP以一个细胞周期依赖的方式结合微管蛋白,并且与细胞分裂纺锤体有关联。此外,TCTP与肌动蛋白细胞骨架相互作用去调节细胞形态 (3)。在有丝分裂中,TCTP蛋白通过PLK激酶使Ser46位点磷酸化,这减少微管的稳定性(4,5)。TCTP蛋白有小GTPase Rheb相互作用,很可能作为一个GEF,因此激活TORC1通路,从而控制细胞生长和增殖(6,7)。TCTP已经证实是涉及凋亡和细胞应激(8-11)。在培养的细胞中,TCTP蛋白表达的减少能引起癌症逆转 (12)。

  1. MacDonald, S.M. et al. (1995) Science 269, 688-90.
  2. Gachet, Y. et al. (1999) J Cell Sci 112 ( Pt 8), 1257-71.
  3. Bazile, F. et al. (2009) Carcinogenesis 30, 555-65.
  4. Yarm, F.R. (2002) Mol Cell Biol 22, 6209-21.
  5. Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.
  6. Dong, X. et al. (2009) J Biol Chem 284, 23754-64.
  7. Hsu, Y.C. et al. (2007) Nature 445, 785-8.
  8. Susini, L. et al. (2008) Cell Death Differ 15, 1211-20.
  9. Bommer, U.A. et al. (2009) Oncogene [Epub ahead of print].
  10. Gnanasekar, M. et al. (2009) Biochem Biophys Res Commun 386, 333-7.
  11. Gnanasekar, M. et al. (2009) Int J Oncol 34, 1241-6.
  12. Tuynder, M. et al. (2002) Proc Natl Acad Sci USA 99, 14976-81.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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