Cell Signaling Technology

Product Pathways - Angiogenesis

HGF β (D6S7D) XP® Rabbit mAb #52445

Hepatocyte growth factor   HGF   sc-13087   Scatter Factor  

No. Size Price
52445S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
52445 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 35, 85 Rabbit IgG
IHC-P 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

HGF β (D6S7D) XP® Rabbit mAb recognizes endogenous levels of total HGF protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human HGF protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human infiltrating ductal carcinoma of the breast HGF β (D6S7D)

XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human embryonal rhabdomyosarcoma using HGF β (D6S7D) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human uterine rhabdomyosarcoma using HGF β (D6S7D) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded A-204 (left) and HeLa (right) cell pellets using HGF β (D6S7D) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of recombinant human HGF (rhHGF) and extracts from A-204 and HeLa cells using HGF β (D6S7D) Rabbit mAb (upper) and β-Actin (D6A8) XP® Rabbit mAb #8457 (lower).

Background

The Hepatocyte Growth Factor (HGF, also known as Scatter Factor) was initially discovered as a mitogenic cytokine that induced hepatocyte replication and proliferation (1-3). HGF is produced by stromal cells where it is processed by extracellular serine proteases into a heterodimer consisting of alpha and beta subunits (4). Through activation of its receptor, cMET, HGF has a wide range of effects beyond hepatocytes that includes angiogensis, epithelial cell proliferation and morphogenesis, and tissue protection and regeneration (5). The HGF-cMET axis has been associated with several diseases, including cancer, where HGF has been shown to promote invasion, metastasis, and drug resistance (6,7). These research studies suggest that HGF is a potential diagnostic and therapeutic target.

  1. Nakamura, T. et al. (1984) Biochem Biophys Res Commun 122, 1450-9.
  2. Russell, W.E. et al. (1984) J Cell Physiol 119, 183-92.
  3. Gohda, E. et al. (1988) J Clin Invest 81, 414-9.
  4. Kataoka, H. et al. Cancer Metastasis Rev 22, 223-36.
  5. Nakamura, T. and Mizuno, S. (2010) Proc Jpn Acad Ser B Phys Biol Sci 86, 588-610.
  6. Matsumoto, K. and Nakamura, T. (2006) Int J Cancer 119, 477-83.
  7. Yano, S. et al. (2008) Cancer Res 68, 9479-87.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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XP is a registered trademark of Cell Signaling Technology, Inc.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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