Cell Signaling Technology

Product Pathways - Screening Technologies

Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb #5243

KinomeView   Motif antibody   PhosphoScan   PTMScan  

No. Size Price
5243S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5243 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Rabbit IgG
IP 1:100
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb detects endogenous levels of cellular proteins containing the Ser-phospho-Thr-Pro motif. This antibody has minor cross-reactivity with phospho-Thr-Pro motifs.

Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb识别内源性的含Ser-phospho-Thr-Pro基序的肽段。此抗体与磷酸化苏氨酸-脯氨酸基序有很弱的交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with an SpTP peptide library.

该单克隆抗体用SpTP肽段库免疫动物制备。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or nocodazole-treated (100 ng/ml, 16 hrs), using Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb.

使用Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb对来自未处理或100ng/ml Nocodazole处理16小时的Hela细胞抽提液进行Western Blot分析。

IP

IP

Immunoprecipitation of extracts from HeLa cells, untreated or nocodazole-treated (100 ng/ml, 16 hrs), using Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb (lanes 1 and 2). 10% input is shown in lanes 3 and 4. Western blots were performed using Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb (upper), RIP (D94C12) XP® Rabbit mAb #3493 (upper middle), MEK1/2 Antibody #9122 (lower middle), and NPM Antibody #3542 (lower).

使用Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb(列1和列2)对来自未处理或100ng/ml Nocodazole处理16小时的Hela细胞抽提液进行免疫共沉淀分析。10%输入显示在列3和4。使用Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb(上)、RIP (D94C12) XP庐 Rabbit mAb #3493(上中)、或MEK1/2 Antibody #9122(中下)和NPM Antibody #3542(下)进行Western blot分析。

Background

Polo-like kinases (PLKs) are Ser/Thr protein kinases that play an essential role during the cell cycle. At least four PLKs exist in mammalian cells: PLK1, PLK2, PLK3, and PLK4 (1). PLKs have a highly conserved amino-terminal kinase domain and a relatively divergent carboxy-terminal domain called the Polo-box domain (PBD). Of the four PLKs, PLK1 is the best characterized (2). PLK1 functions as a key regulator of mitotic events by phosphorylating substrate proteins on centrosomes, kinetochores, the mitotic spindle, and the midbody, and is crucial for proper progression through multiple stages of mitosis (2-4). The PBDs of PLK1 function as a phospho-Ser/Thr-binding module, recognizing the optimal recognition sequence motif Ser-(pSer/pThr)-(Pro/X) (5). Binding of phosphopeptides containing the Ser-(pSer/pThr)-(Pro/X) motif by the PBD in PLK1 relieves its inhibitory function on kinase activity (6). Ser-(pSer/pThr)-Pro peptides are phosphorylated by proline-directed kinases such as cyclin dependent kinases (CDKs). These findings imply that priming phosphorylations on substrates or docking proteins by other mitotic kinases such as CDKs may target PLK1 to its substrates and simultaneously activate its kinase activity. Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb is a useful tool to study PLK1 binding proteins and PLK1 substrates.

Polo-like kinases (PLKs)是丝氨酸/苏氨酸激酶,在细胞周期中扮演重要角色。哺乳动物细胞中至少存在四种PLK:PLK1、PLK2、PLK3和PLK4(1)。PLK具有高度保守的氨基末端激酶结构域和一个相对不保守的羧基末端结构域Polo-box域(PBD)。在四种PLK中,PLK1是描述得最清楚的(2)。PLK1作为有丝分裂事件的关键调节者而发挥作用,它可以磷酸化中心体、着丝粒、有丝分裂纺锤体、中心颗粒体的底物蛋白,对有丝分裂多个阶段的正确进展起关键作用(2-4)。PLK1的PBD作为磷酸化丝氨酸/苏氨酸结合基序而发挥作用,识别最优识别基序Ser-(pSer/pThr)-(Pro/X)(5)。PLK1的PBD结合包含Ser-(pSer/pThr)-(Pro/X)基序的磷酸化肽段后解除其对激酶活性抑制功能(6)。Ser-(pSer/pThr)-Pro肽被脯氨酸导向的激酶如细胞周期依赖激酶(CDK)磷酸化。这些发现提示其它有丝分裂激酶如CDK对底物或接合蛋白可能是通过PLK1和其底物作用而同时激活其激酶活性而促发的。Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb是研究PLK1结合蛋白和PLK1底物的有用工具。

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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