Cell Signaling Technology

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Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) #5232

No. Size Price
5232S 100 µl ( 10 immunoprecipitations ) ¥3,580.00 现货查询 购买询价
5232 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
ChIP 1:50 Human, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: ChIP=Chromatin IP,

Specificity / Sensitivity

Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) detects endogenous levels of total nanog protein. Nanog (D73G4) XP® Rabbit mAb 兔单抗(ChIP级别)识别内源性的总nanog蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human nanog protein.

此单克隆抗体由合成肽段免疫动物产生,该肽段与人nanog蛋白邻近氨基末端的氨基酸序列一致。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 10 μl of Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.4 x 106 NCCIT细胞细胞染色质与SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003的10μl Nanog (D73G4) XP®兔源单克隆抗体(ChIP级别)或2μl正常兔IgG#2729 交联。富集到的DNA随后使用SimpleChIP® Human Oct-4启动子引物#4641,SimpleChIP® Human Sox2启动子引物#4649和SimpleChIP®人α卫星重复序列引物#4486,进行荧光实时PCR定量。各样品沉淀得到的DNA量通过与input染色质总量对比获得,相当于1份.4 x 106 NTERA-2细胞。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NTERA-2 cells and 10 μl of Nanog, Oct-4 and Sox2 antibodies, or 2 μl of Normal Rabbit IgG, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human Nanog promoter primers, SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106 NCCIT细胞染色质与10μl Nanog, Oct-4和Sox2抗体或2 μl正常兔IgG交联。富集到的DNA随后使用人Nanog启动子引物,SimpleChIP®人Oct-4启动子引物#4641,SimpleChIP®人Sox2启动子引物#4649和SimpleChIP®人α卫星重复序列引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量通过与input染色质总量对比获得,相当于1份.4 x 106 NTERA-2细胞。

Background

Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and Lin28 (2,3). Nanog是一类同源结构域包含转录因子,为胚胎干细胞多潜能性和自我更新所必需(1) Nanog的表达受Sox2、多潜能性调节关键因子Oct-4(1)。最近关于体细胞程序化重编的进展用到了包括nanog, Oct-4, Sox2, KLF4, c-Myc, and Lin28在内的转录因子协同的病毒表达(2,3)。

  1. Kim, J. et al. (2008) Cell 132, 1049-61.
  2. Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9.
  3. Yu, J. et al. (2007) Science 318, 1917-20.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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