Cell Signaling Technology

Product Pathways - NF-kB Signaling

Phospho-CARD11 (Ser652) Antibody #5189

No. Size Price
5189S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5189 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 130 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

Phospho-CARD11 (Ser652) Antibody detects endogenous levels of CARD11 protein only when phosphorylated at Ser652. This site corresponds to Ser671 in mouse and Ser668 in rat CARD11 proteins.

Phospho-CARD11 (Ser652) Antibody 只能检测内源的在Ser652位点磷酸化的CARD11蛋白。此位点分别对应于与小鼠 ARD11蛋白的Ser671位点和大鼠CARD11蛋白的Ser668位点。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to a region surrounding Ser652 of human CARD11 protein. Antibodies were purified by protein A and peptide affinity chromatography.

此多克隆抗体是通过合成人源对应的CARD11 Ser652位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or treated with TPA #9905 (40 nM) and calcium ionophore A23187 (2 μM) for 15 minutes, and λ-phosphatase plus calf intestinal phosphatase (CIP) as indicated, using Phospho-CARD11 (Ser652) Antibody (upper) and CARD11 (1D12) Rabbit mAb #4435 (lower).Western免疫印迹。用Phospho-CARD11 (Ser652) Antibody (上图) 和CARD11 (1D12) Rabbit mAb #4435 (下图)研究未经处理的和经 TPA #9905 (40 nM) ,钙离子载体A23187 (2 μM) 15 min, λ-磷酸酶和小牛碱性磷酸酶(CIP)处理的Jurkat细胞的细胞提取液。


CARD11/Carma1/Bimp3 belongs to the MAGUK (membrane-associated guanylate kinase) family that typically function as molecular scaffolds in the assembly of multiprotein complexes (1,2). MAGUK family members contain an SH3 domain, a PDZ domain and a GuK domain homologous to guanylate kinase. In addition, CARD11 contains an amino-terminal CARD domain (caspase recruitment domain). This domain plays an important role in forming interactions with a number of proteins containing CARD domains that are involved in regulating apoptosis and NF-κB activation. CARD11 is predominately expressed in lymphocytes (1,2) and associates with the CARD domain of Bcl10. When overexpressed, CARD11 leads to the phosphorylation of Bcl10 and activation of NF-κB (1,2). CARD11 is constitutively associated with lipid rafts and is thought to function by recruiting Bcl10 and MALT1 and triggering the phosphorylation of IKKs (3,4). Several studies using the genetic disruption of CARD11 or dominant-negative mutations have demonstrated that it plays a critical role in NF-κB activation and lymphocyte signaling (4-7).

CARD11/Carma1/Bimp3属于MAGUK (膜相关的鸟苷酸激酶) 家族,其典型的功能是为多蛋白复合体的组转提供分子支架(1,2)。MAGUK家族成员包含一个SH3域,一个PDZ 域和一个GuK域并与鸟苷酸激酶同源。另外,CARD11包含一个N-末端CARD 域(蛋白酶级联反应招募域)。此结构域在与很多含有CARD域的蛋白相互作用来调控凋亡和NF-κB激活过程中发挥了重要的作用。CARD11主要在淋巴细胞中表达 (1,2)并与Bcl10的CARD域相关。过表示时,CARD11 导致了 Bcl10的磷酸化和NF-κB的激活(1,2)。CARD11构成上与脂质有关在功能上能够招募Bcl10和MALT1并引起 IKKs的磷酸化(3,4)。一些利用遗传学方法破坏CARD11或采用显性负性突变的研究表明它在NF-κB的激活和淋巴细胞的信号中起了重要的作用(4-7)。

Phosphorylation at multiple sites within the central region of CARD11 regulates NF-κB activation (8-10).

在CARD11中间区域的多位点磷酸化能调控NF-κB 的激活(8-10)。

  1. Bertin, J. et al. (2001) J. Biol. Chem. 276, 11877–11882.
  2. Gaide, O. et al. (2001) FEBS Lett. 496, 121-127.
  3. Stilo, R. et al. (2004) J. Biol. Chem. 279, 34323-34331.
  4. Wang, D. et al. (2002) Nat. Immunol. 3, 830-835.
  5. Jun, J.E. et al. (2003) Immunity 18, 751-762.
  6. Hara, H. et al. (2003) Immunity 18, 763-775.
  7. Gaide, O. et al. (2002) Nat. Immunol. 3, 836-843.
  8. Sommer, K. et al. (2005) Immunity 23, 561-74.
  9. Shinohara, H. et al. (2007) J Exp Med 204, 3285-93.
  10. Moreno-García, M.E. et al. (2009) J Immunol 183, 7362-70.

Application References

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