Cell Signaling Technology

Product Pathways - Transcription Factors

TRIM29/ATDC Antibody #5182

No. Size Price
5182S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
5182 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 66 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

TRIM29/ATDC Antibody detects endogenous levels of TRIM29/ATDC protein.

TRIM29/ATDC Antibody能够检测内源性TRIM29/ATDC蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu160 of human TRIM29/ATDC protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from SW480, SW620, and HT-29 cells using TRIM29/ATDC Antibody.

使用TRIM29/ATDC Antibody,免疫印迹(Western blot)分析SW480、SW620和HT-29细胞中TRIM29/ATDC的蛋白水平。


Tipartite motif-containing protein 29 (TRIM29 or ATDC) was isolated as a candidate gene by its ability to complement the radiosensitivity defect of an ataxia-telangiectasia (AT) cell line (1). This putative transcription regulator belongs to the TRIM (tripartite motif) protein family that is characterized by highly conserved amino-terminal RING finger, B-box, and coiled-coil domains. TRIM29 binds and sequesters cytosolic p53, repressing expression of p53 target genes including p21 and Noxa by preventing p53 from entering the nucleus. Expression of TRIM29 inhibits p53 function and results in increased cell proliferation. (2). TRIM29 enhances tumor growth and metastasis in vivo and high TRIM29 levels are seen in most invasive pancreatic cancers. The oncogenic effect of TRIM29 appears to require β-catenin as expression of both proteins is elevated in pancreatic cancer cell lines and tissues (3).

Tipartite motif-containing protein 29 (TRIM29 or ATDC)被分离作为一个候选基因,该基因能够互补ataxia-telangiectasia (AT)细胞系的辐射敏感度缺陷(1)。该假定的转录因子属于TRIM (tripartite motif)蛋白家族,该家族是以高度保守的氨基端RING finger、B-box和coiled-coil为特征。TRIM29结合和隔绝细胞质p53,这可通过阻断p53进入细胞核从而抑制p53靶基因的表达包括p21和Noxa。TRIM29蛋白的表达可抑制p53功能,并且导致细胞增殖的增加(2)。TRIM29能够提高肿瘤生长和体内转移,并且TRIM29蛋白高表达在许多侵入性胰腺癌中被发现。TRIM29的致癌效应似乎需要β-catenin,因为这两个蛋白表达在胰腺癌细胞系和组织中提高(3)。

  1. Kapp, L.N. et al. (1992) Am J Hum Genet 51, 45-54.
  2. Yuan, Z. et al. (2010) Mol Cell Biol 30, 3004-15.
  3. Wang, L. et al. (2009) Cancer Cell 15, 207-19.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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