Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-CD79A (Tyr182) Antibody #5173

CD79  

No. Size Price
5173S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5173T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
5173 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 45-55 Rabbit
IP 1:50
F 1:100
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-CD79A (Tyr182) Antibody detects endogenous levels of CD79A protein only when phosphorylated on Tyr182 of CD79A.

Phospho-CD79A (Tyr182)能够检测在Tyr182位点磷酸化的内源CD79A蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr182 of Mouse CD79A protein. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆抗体是通过合成小鼠源对应的CD79A Tyr182位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Ramos cells, untreated (blue) or IgM-treated (green), using Phospho-CD79A (Tyr182) Antibody.流式细胞仪分析未经处理的(蓝色)和经IgM处理的(绿色)Ramos细胞,所用抗体为Phospho-CD79A (Tyr182) Antibody。

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos cells untreated or treated with anti-human IgM (12 µg/ml for 10 minutes), using Phospho-CD79A (Tyr182) Antibody (upper) or CD79A Antibody #3351 (lower).Western免疫印迹。用 Phospho-CD79A (Tyr182) Antibody (上图) 或 CD79A Antibody #3351 (下图)研究未经处理的和经anti-human IgM (12 µg/ml,10 min)处理的Ramos细胞的细胞提取液。

IF-IC

IF-IC

Confocal immunofluorescent analysis of Ramos cells, untreated (left) or IgM-treated (middle), and Jurkat cells (right) using Phospho-CD79A (Tyr182) Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析Ramos细胞,未经处理 (左图),经IgM处理的(中间图)和Jurkat细胞(右图),所用抗体为Phospho-CD79A (Tyr182) Antibody(绿色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。

Background

Antigen receptors found on the surface of B-cells contain a hetrodimeric signaling component composed of CD79A and CD79B, also known as Ig α and Ig β, respectively (1,2). Presence of this receptor complex is essential for B-cell development and function (3). Together these two proteins and the associated B cell receptor initiate intracellular signaling following antigen binding (4,5). An immunoreceptor tyrosine-based activation motif (ITAM) is found in the CD79A intracellular region appears to be is important for its function (6). Antigen binding precedes formation of the CD79A and CD79B heterodimer and subsequent activation of receptor associated kinases (7). CD79A is a marker for B-lineage lymphoblastic leukemia (8); mutations in the corresponding mb-1 gene are responsible for abnormally low levels of functional B cell receptors in some cases of chronic B-cell lymphocytic leukemia (9).

B细胞上的抗原受体包含了异源二聚体信号成员,此二聚体由CD79A 和CD79B组成,也分别称作是Ig α和Ig β(1,2)。此受体复合体的存在对B细胞的发育和功能有着重要的意义(3)。抗原结合后,这两个蛋白和B细胞相关的受体一起起始胞内的信号转导T(4,5)。一个免疫受体酪氨酸相关的激活区域(ITAM)存在于CD79A胞内区域,并对其功能发挥了重大的作用(6)。抗原结合,然后形成CD79A 和 CD79B异源二聚体,最后激活受体相关的激酶(7)。CD79A是B细胞系成淋巴细胞的白血病的一个特征分子(8); 在有些慢性B细胞淋巴细胞白血病中,对应mb-1基因的突变对异常低水平的功能性B细胞受体有重要的作用(9)。

Tyr182 of CD79A can be phosphorylated by Src family kinases Lyn and Blk (10).

CD79A的Tyr182位点能够被Src家族激酶Lyn和Blk磷酸化(10)。

  1. van Noesel, C.J. et al. (1991) J Immunol 146, 3881-8.
  2. Minegishi, Y. et al. (1999) J Clin Invest 104, 1115-21.
  3. Yu, L.M. and Chang, T.W. (1992) J Immunol 148, 633-7.
  4. Storch, B. et al. (2007) Eur J Immunol 37, 252-60.
  5. Mason, D.Y. et al. (1995) Blood 86, 1453-9.
  6. Luisiri, P. et al. (1996) J Biol Chem 271, 5158-63.
  7. Pike, K.A. et al. (2004) J Immunol 172, 2210-8.
  8. Astsaturov, I.A. et al. (1996) Leukemia 10, 769-73.
  9. Vuillier, F. et al. (2005) Blood 105, 2933-40.
  10. Pao, L.I. et al. (1998) J Immunol 160, 3305-14.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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