Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-MYPT1 (Thr696) Antibody #5163

MBS   MYOSIN PHOSPHATASE   Myosin phosphatase target subunit 1   MYOSIN-BINDING SUBUNIT   PPP1R12A   PROTEIN PHOSPHATASE 1   REGULATORY SUBUNIT 12A   sc-17556  

No. Size Price
5163S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5163T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
5163 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 140 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-MYPT1 (Thr696) Antibody detects endogenous levels of MYPT1 only when phosphorylated at threonine 696.

Phospho-MYPT1 (Thr696) Antibody仅检测threonine 696位点磷酸化的内源性MYPT1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surroundiing Thr696 of human MYPT1. Antibodies are purified using protein A and peptide affinity chromatography.

通过人工合成人源MYPT1蛋白Thr696位点周围相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untreated or treated with the ROCK inhibitor Y27632 (2 minutes at 10 μM) using Phospho-MYPT1 (Thr696) Antibody or total MYPT1 Antibody #2634. Phospho-MYPT1 (Thr696) Antibody was pre-incubated as indicated with phospho-MYPT1 (Thr696) peptide or nonphospho-MYPT1 (Thr696) peptide.

使用Phospho-MYPT1 (Thr696) Antibody或MYPT1 Antibody #2634,免疫印迹(Western Blot)分析293细胞中Phospho-MYPT1(Thr696)和MYPT1蛋白水平,细胞分为未处理和ROCK inhibitor Y27632 (2 minutes at 10 μM)处理。Phospho-MYPT1 (Thr696) Antibody提前和phospho-MYPT1 (Thr696) peptide或nonphospho-MYPT1 (Thr696) peptide孵育。

Background

Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions. Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c). It is estimated that over fifty different regulatory subunits exist (1). 
 The myosin phosphatase holoenzyme is composed of three subunits: the PP1 catalytic subunit (PP1c), a targeting/regulatory subunit (MYPT/myosin-binding subunit of myosin phosphatase) and a 20 kDa subunit of unknown function (M20). MYPT binding to PP1cδ alters the conformation of the catalytic cleft and increases enzyme activity and specificity (2). Two MYPT isoforms that are 61% identical have been described. MYPT1 is widely expressed while MYPT2 expression appears to be exclusive to heart and brain (3). Related family members also include MBS85, MYPT3 and TIMAP (4). 
 Myosin phosphatase regulates the interaction of actin and myosin in response to signaling through the small GTPase Rho. Rho activity inhibits myosin phosphatase via Rho-associated kinase (ROCK). Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization (5,6).

Protein phosphatase 1 (PP1)是一个普遍存在的真核蛋白质丝氨酸/苏氨酸磷酸酶,其涉及不同细胞功能的调节。底物的特异性通过一个调节亚单位与PP1 catalytic subunit (PP1c)的结合被确定。据估计大约有五十个不同的调节亚单位存在(1)。myosin 磷酸酶全酶有三个亚单位组成:PP1催化亚单位(PP1c) 、一个靶向/调节亚单位(MYPT/myosin-binding subunit of myosin phosphatase)和一个未知功能的20 kDa大小的亚单位 (M20)。MYPT蛋白结合到PP1cδ蛋白可改变催化部位的构象以及子女国家酶活性和特异性 (2)。61%序列相同的两个MYPT亚单位已经被描述。MYPT1蛋白广泛表达,而MYPT2 蛋白的表达只表达在心脏和大脑组织(3)。相关的家族成员也诱导MBS85、MYPT3和TIMAP (4)。在小GTPase Rho的信号下,Myosin磷酸酶调节actin和myosin相互作用。通过Rho-associated kinase (ROCK)作用,Rho活性抑制myosin磷酸酶。MYPT1蛋白在Thr696和Thr853位点的磷酸化导致磷酸酶抑制和细胞骨架重组(5,6)。

  1. Cohen, P.T. (2002) J Cell Sci 115, 241-56.
  2. Terrak, M. et al. (2004) Nature 429, 780-4.
  3. Fujioka, M. et al. (1998) Genomics 49, 59-68.
  4. Ito, M. et al. (2004) Mol Cell Biochem 259, 197-209.
  5. Birukova, A.A. et al. (2004) Microvasc Res 67, 64-77.
  6. Birukova, A.A. et al. (2004) J Cell Physiol 201, 55-70.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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