Cell Signaling Technology

Product Pathways - DNA Damage

Phospho-ATRIP (Ser224) Antibody #5161

ATM and Rad3-related-interacting protein   ATR-interacting protein  

No. Size Price
5161S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5161 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 86 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Monkey,

Specificity / Sensitivity

Phospho-ATRIP (Ser224) Antibody detects endogenous levels of ATRIP protein only when phosphorylated at Ser224. Phospho-ATRIP (Ser224)抗体能够检测内源性丝氨酸(224位点)磷酸化的ATRIP蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phoshopeptide corresponding to residues surrounding Ser224 of the human ATRIP protein. 该多克隆抗体是由合成的人源的针对ATRIP蛋白近丝氨酸(224位)的磷酸化肽段免疫动物生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, untreated or treated with λ and calf intestinal phosphatases, using Phospho-ATRIP (Ser224) Antibody (upper) or ATRIP Antibody #2737 (lower). Western blot方法检测细胞提取物:未处理和λ 以及牛小肠磷酸酶处理的293T细胞,使用的抗体为Phospho-ATRIP (Ser224)抗体(上图)和 ATRIP 抗体 #2737(下图)。

Background

In response to genomic stress, the ATR interacting protein (ATRIP) binds and is phosphorylated by the DNA damage-and checkpoint-activated kinase ATR (ataxia-telangiectasia mutated and rad3-related). Both ATR and ATRIP are integral for checkpoint signaling and are critical in the DNA repair response (1-3). Direct interaction between ATRIP and replication protein A (RPA) at RPA-coated, single-stranded DNA results in the recruitment of phosphorylated ATR/ATRIP to stalled replication forks and sites of DNA damage (3). ATR/ATRIP coordinate DNA repair and cell cycle progression in conjunction with key regulatory proteins, such as Rad17 and the 9-1-1 complex (4). ATR associated with ATRIP can also be stimulated by topoisomerase II binding protein (TOPBP1), suggesting that ATRIP may regulate both ATR localization and activity (5). 在基因组应激中,ATR相互作用蛋白(ATRIP)结合并被DNA损伤和检验点激活激酶ATR(共济失调毛细血管扩张症兼Rad3相关激酶)磷酸化。ATR和ATRIP在检验点信号通路中是一体的并在DNA修复反应中起重要作用(1-3)。RPA包被的单链DNA处ATRIP和RPA (复制蛋白A)的直接相互作用引起磷酸化ATR/ATRIP招募从而导致复制叉停滞和DNA损伤(3)。ATR/ATRIP协同重要的调节蛋白如Rad17 和9-1-1 复合物调整DNA修复和细胞周期进程(4)。TOPBP1(拓扑异构酶II结合蛋白)可以激活ATR与ATRIP的相互作用,这表明ATRIP可能调节ATR的定位和活性(5)。

Cyclin dependent kinase 2 (CDK2) may participate in the regulation of DNA damage response and cell cycle control through phosphorylation of ATRIP at Ser224 (6). 细胞周期依赖性蛋白激酶2(CDK2)可通过ATRIP蛋白丝氨酸(224位点)磷酸化参与DNA损伤反应和细胞周期控制的调节。

  1. Cortez, D. et al. (2001) Science 294, 1713-1716.
  2. Itakura, E. et al. (2004) Biochem. Biophys. Res. Commun. 323, 1197-1202.
  3. Zou, L. and Elledge, S.J. (2003) Science 300, 1542-1548.
  4. Yang, X.H. and Zou, L. (2006) Methods Enzymol. 409, 118-131.
  5. Ball, H.L. et al. (2007) Mol Cell Biol 27, 3367-77.
  6. Myers, J.S. et al. (2007) Cancer Res 67, 6685-90.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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