Cell Signaling Technology

Product Pathways - MAPK Signaling

SRF (D71A9) XP® Rabbit mAb #5147


No. Size Price
5147S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
5147T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
5147 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,Pig, Endogenous 67 Rabbit IgG
IP 1:50
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

SRF (D71A9) XP® Rabbit mAb recognizes endogenous levels of total SRF protein.

SRF (D71A9) XP® Rabbit mAb兔单抗能检测内源性总SRF蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser375 of human SRF protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SRF (D71A9) XP® Rabbit mAb.western blot方法检测细胞提取物:各种细胞系,使用的抗体是SRF (D71A9) XP® Rabbit mAb。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells starved overnight and treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (100 ng/ml) for 3 hours, and either 5 μl of SRF1 (D71A9) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Fos Promoter Primers #4663 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫沉淀反应中使用的是无培养基过夜和TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (100 ng/ml)处理3 h后的4 x 106HeLa细胞中交联的染色质,使用的抗体是5 μl SRF1 (D71A9) XP® Rabbit mAb和2 μl Normal Rabbit IgG #2729,试剂盒是SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003。real-time PCR定量DNA的量,使用的引物是SimpleChIP® Human c-Fos Promoter Primers #4663和SimpleChIP® Human α Satellite Repeat Primers #4486。每个样品中免疫沉淀DNA的量相当于加入总染色质的量。


The serum response factor (SRF) is a 67 kDa phospho-protein that, together with auxiliary factors, modulates transcription of immediate early genes containing serum response elements at their promoters (1,2). SRF contains several phosphorylation sites (3), but functional consequences of phosphorylation have not been identified unequivocally. Several growth factor- and calcium-regulated kinases, such as p90RSK and CaM kinase IV, can phosphorylate SRF at Ser103 (4,5), and Ser103 of SRF is also a nuclear target for MAPKAP kinase 2 (6).

血清效应因子(SRF) 是67 kDa的磷酸化蛋白,它与辅助因子一起修饰即时早期基因的转录,这些基因的启动子中包含血清效应元件(1,2)。SRF包含一些磷酸化位点(3),但是磷酸化的功能性序列目前还不明确。一些生长因子和钙调节激酶,如p90RSK、CaM kinase IV,能磷酸化SRF蛋白的丝氨酸103位点(4,5),SRF蛋白的丝氨酸103位点是MAPKAP激酶2的细胞核上靶点(6)。

  1. Treisman, R. (1995) EMBO J. 14, 4905-4913.
  2. Janknecht, R. et al. (1995) Carcinogenesis 16, 443-450.
  3. Marais, R. M. et al. (1992) EMBO J. 11, 97-105.
  4. Rivera, V. M. et al. (1993) Mol. Cell. Biol. 13, 6260-6273.
  5. Miranti, C. K. et al. (1995) Mol. Cell. Biol. 15, 3672-3684.
  6. Heidenreich, O. et al. (1999) JBC 274, 14434-14443.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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