Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Cyclophilin A (D2Y4M) Rabbit mAb #51418

No. Size Price
51418S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
51418 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 18 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Cyclophilin A (D2Y4M) Rabbit mAb recognizes endogenous levels of total cyclophilin A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly18 of mouse cyclophilin A protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Cyclophilin A (D2Y4M) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extract from C2C12 cells, mock transfected (-) or transfected with siRNA against mouse cyclophilin A (+), using Cyclophilin A (D2Y4M) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower)

Background

Cyclophilins are a highly conserved family of peptidylprolyl cis-trans-isomerases (PPIA) that are targets of the immunosuppressant drug cyclosporin A (CsA) (1,2). The complex of cyclophilin and CsA can bind to and inhibit calcineurin which leads to inhibition of the transcription factor NFAT and decreased production of cytokines (3,4). As isomerases, cyclophilins have been proposed to aid in protein folding. Cyclophilin A can bind to the p55 Gag protein of HIV and appears necessary for HIV infection (5,6). There is also some evidence that cyclophilins have nuclease activity and play a role in apoptosis (7).

  1. Takahashi, N. et al. (1989) Nature 337, 473-475.
  2. Wang, P. and Heitman, J. (2005) Genome Biol. 6, 226.
  3. Jain, J. et al. (1993) Nature 365, 352-355.
  4. McCaffrey, P.G. et al. (1993) J. Biol. Chem. 268, 3747-3752.
  5. Thali, M. et al. (1994) Nature 372, 363-365.
  6. Streblow, D.N. et al. (1998) Virology 245, 197-202.
  7. Montague, J.W. et al. (1994) J. Biol. Chem. 269, 18877-18880.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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