Cell Signaling Technology

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Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127

anti rabbit   anti-rabbit   sc-2054   secondary  

No. Size Price
5127S 100 µl ( 20 western blots ) ¥2,194.00 现货查询 购买询价
5127 carrier free & custom formulation / quantityemail request
Applications Species-Reactivity Sensitivity MW (kDa) Isotype
W Endogenous Mouse

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) recognizes the native rabbit IgG. It does not recognize the denatured and reduced rabbit IgG heavy (about 50 kDa) or light (about 25 kDa) chains on western blot.

Mouse Anti-rabbit IgG (构象特异性) (L27A9) mAb (HRP 标记)识别天然的兔源IgG.不能与变性的和缺少重链(50KDa)和轻链(25KDa)的兔源IgG结合。

Source / Purification

Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.

单克隆抗体是通过采用天然的全兔IgG免疫动物生产的。

IP

IP

Immunoprecipitation of PRAS40 from HeLa cells using PRAS40 (D23C7) XP® Rabbit mAb #2691 (lane 2). Lane 1 contains input control lysate. All lanes are probed with PRAS40 (D23C7) Rabbit mAb #2691 as the primary antibody. Secondary antibodies include Anti-rabbit IgG, HRP-linked Antibody #7074 (panel A) and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 (panel B).

IP

IP

Immunoprecipitation of Beclin-1 from HeLa cells using Beclin-1 (D40C5) Rabbit mAb #3495 (lane 2). Lane 1 contains input control lysate. All lanes are probed with Beclin-1 (D40C5) Rabbit mAb #3495 as the primary antibody. Secondary antibodies include Anti-rabbit IgG, HRP-linked Antibody #7074 (panel A) and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 (panel B).免疫沉淀分析HeLa细胞的Beclin-1 ,所用检测抗体为Beclin-1 (D40C5) Rabbit mAb #3495 (泳道2),泳道1包含对照组的提取物。所由泳道采用Beclin-1 (D40C5) Rabbit mAb #3495 作为一抗。二抗包括Anti-rabbit IgG, HRP-linked Anti- body #7074 (图 A) 和 Mouse Anti-rabbit IgG (构象特异性) (L27A9) mAb (HRP 标记) #5127( 图B).

Background

Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb only reacts with native IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb as a secondary antibody will eliminate this problem.

Mouse Anti-rabbit IgG (构象特异性) (L27A9) mAb (HRP 标记)仅与天然IgG发生反应,不能与变性还原的兔源IgG的重链和轻链结合。当免疫沉淀后进行western blotting时,一抗结合的变性的兔源IgG轻链和重链的位置大约分别在25和50KDa,在随后的western blot中,会有相同分子量的条带模糊的蛋白。采用 Mouse Anti-rabbit IgG (构象特异性) (L27A9) mAb (HRP 标记)了一减少这种问题的发生。

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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