Cell Signaling Technology

Product Pathways - Neuroscience

TPH2 (D3E5I) XP® Rabbit mAb #51124

No. Size Price
51124S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
51124 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 52 Rabbit IgG
IF-F 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

TPH2 (D3E5I) Rabbit mAb recognizes endogenous levels of total TPH2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu30 of human TPH2 protein.

IF-F

IF-F

Confocal immunofluorescent analysis of the ventral tegmental region of adult mouse brain using TPH2 (D3E5I) XP® Rabbit mAb (green). Red = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human TPH2 (hTPH2; +) using TPH2 (D3E5I) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from rat brain and rat ventral tegmental region using TPH2 (D3E5I) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of serotonin (1) by converting tryptophan to 5-hydroxy-L-tryptophan (2). Two isoforms of TPH exist: TPH-1 is mainly expressed in the periphery, whereas the expression of TPH-2 is restricted to neuronal cells and the central nervous system (3). Most of the serotonin found throughout the body is synthesized by TPH-1 in enterochromaffin cells of the gastrointestinal tract. Targeted disruption of the tph1 gene results in low levels of circulating and tissue serotonin (4).

Polymorphisms and regulation of the tph2 gene are involved in cognitive, emotional, and psychiatric disorders (5-7)

  1. Hale, M.W. et al. (2011) J Chem Neuroanat 41, 219-26.
  2. Silva, S.R. et al. (2011) Anticancer Res 31, 1153-60.
  3. Walther, D.J. et al. (2003) Science 299, 76.
  4. Amireault, P. et al. (2011) Proc Natl Acad Sci U S A 108, 13141-6.
  5. Waider, J. et al. (2011) Psychoneuroendocrinology 36, 393-405.
  6. Choong, M.Y. et al. (2014) Psychiatry Res 220, 1163-6.
  7. Zhang, Y. et al. (2015) Mol Med Rep 12, 3184-90.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications