Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb #5112

Activating Transcription Factor 2   atf   atf2   phospho-atf  

No. Size Price
5112S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
5112T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
5112 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 70 Rabbit IgG
F 1:1600

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry,

Specificity / Sensitivity

Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb detects endogenous levels of ATF-2 only when phosphorylated at threonine 71. This antibody does not cross-react with phosphorylated c-Jun, CREB or other transcription factors. It recognizes both Thr69/Thr71 dually phosphorylated ATF-2 and Thr71 singly phosphorylated ATF-2 equally well.

Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb兔单抗能够检测Thr71位点磷酸化的内源性ATF-2总蛋白水平,不能与磷酸化的c-Jun、CREB蛋白和其他转录因子发生交叉反应。它能识别Thr69/71位点共磷酸化的ATF-2,以及Thr71位点单磷酸化的ATF-2蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF2.


Western Blotting

Western Blotting

Western blot analysis of extracts from untreated or UV-treated COS cells, NIH/3T3 cells and C6 cells, using Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (upper), or ATF-2 (20F1) Rabbit mAb #9226 (lower).Western blot 方法检测细胞提取物:未经处理和紫外照射的COS、C6细胞,使用的抗体是Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (上图), or ATF-2 (20F1) Rabbit mAb #9226 (下图)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of THP-1 cells, untreated (blue) or Anisomycin treated (green), using Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb compared to a nonspecific negative control antibody (red).Flow cytometric方法检测细胞提取物:未经处理(蓝色)和茴香霉素处理的(绿色)THP-1 细胞,使用的抗体是Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb,红色表示非特异性阴性对照抗体。


The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).

转录因子ATF-2(也被称为 CRE-BP1)能够结合到AP-1和 CRE DNA反应元件,是ATF/CREB亮氨酸拉链蛋白质家族的成员(1)。ATF-2蛋白能够调控多种病毒癌基因以及抑癌基因的表达,是SAPK/JNK 、p38 MAPK信号传导通路的靶标 (2-4)。多种细胞压力包括基因毒剂、炎性细胞因子和紫外辐射,都能激活ATF-2蛋白的转录活性。细胞应激刺激通过磷酸化Thr69和Thr71位点使ATF-2蛋白被激活 (2-4)。在体外以及转染了ATF-2的细胞中,SAPK、p38 MAPK蛋白都被证明能够磷酸化ATF-2蛋白的这些位点。这些位点的突变能导致应激性的ATF-2诱导转录活性降低(2-4),并且降低E1A 、Rb蛋白通过ATF-2介导的基因表达。(2)。

  1. Abdel-Hafiz, H.A. et al. (1992) Mol Endocrinol 6, 2079-89.
  2. Gupta, S. et al. (1995) Science 267, 389-93.
  3. van Dam, H. et al. (1995) EMBO J 14, 1798-811.
  4. Livingstone, C. et al. (1995) EMBO J 14, 1785-97.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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