Cell Signaling Technology

Product Pathways - Nuclear Receptor Signaling

Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb #5095

early response protein NAK1   NR4A1   nuclear receptor subfamily 4. group A. member 1 {NR4A1}   orphan nuclear receptor Nur77   steroid receptor TR3  

No. Size Price
5095S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5095 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 70-80 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb detects endogenous levels of Nur77 protein only when phosphorylated at Ser351.只有当丝氨酸(351位)磷酸化时,Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb能够检测内源性水平的Nur77蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser351 of human Nur77 protein.该单克隆抗体通过用合成磷酸肽免疫动物制备,该合成磷酸肽是人Nur77蛋白丝氨酸(351位)附近的残基。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or treated with TPA #4174 and calcium ionophore A23187 for the indicated times, using Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb (upper) or Nur77 (D63C5) XP® Rabbit mAb #3960 (lower).Western blot方法检测Jurkat细胞提取物,细胞不处理或用TPA #4174和钙离子载体按指定的时间处理,使用的抗体为Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb (上图)或 Nur77 (D63C5) XP® Rabbit mAb #3960 (下图).

Western blot analysis of extracts from Jurkat cells, untreated or treated with TPA #4174 and calcium ionophore A23187 for 4 hours, using Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb. Antibody phospho-specificity was confirmed by pre-incubating the antibody with either a phosphorylated site-specific peptide to block the signal or a non-phosphorylated site-specific peptide that does not block the signal.Western blot方法检测Jurkat细胞提取物,细胞不处理或用TPA #4174和钙离子载体处理4小时,使用的抗体为Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb. 通过将抗体与磷酸化位点特异性多肽进行预孵育以阻断信号,或非磷酸化的位点特异性多肽不阻断信号,证实了抗体磷酸化的特异性。


Nur77, also known as TR3 and NGFI-B, is an immediate-early response gene and an orphan member of the steroid/thyroid/retinoid receptor superfamily (1-3). Nur77 is composed of an amino-terminal transactivation domain, a central DNA-binding domain and a carboxy-terminal ligand-binding domain. Expression of Nur77 is rapidly induced by a variety of stimuli, including apoptotic, mitogenic and stress signals (1-6). It has been proposed to have many functions related to cell proliferation, differentiation and apoptosis. Nur77 has been extensively studied in T cells where it has been implicated in the process of negative selection and TCR-mediated apoptosis (5,6). Nur77 binds to specific DNA elements leading to the regulation of target genes (7). As a possible mechanism for regulating apoptosis, Nur77 can induce the expression of apoptotic genes such as FasL and TRAIL (8,9). Nur77 is heavily phosphorylated by multiple kinases, which may affect its transactivation activity as well as its subcellular localization (4,10,11). Translocation of Nur77 from the nucleus to the mitochondria can regulate its association with Bcl-2 and control the release of cytochrome c, thereby triggering apoptosis (12,13).Nur77也称为TR3和NGFI-B,是一种即早反应基因和类固醇/甲状腺/类视黄醇受体超家族的孤儿成员(1-3)。 Nur77由一个氨基末端反式激活域,一个中央DNA结合结构域和一个羧基-末端配体结合域组成。Nur77的表达由多种刺激快速诱导,包括细胞凋亡、有丝分裂和应激信号(1-6)。已经提出有多个与细胞增殖、分化和细胞凋亡相关的功能。在T细胞中,Nur77已被广泛研究,它与负选择过程和TCR介导的细胞凋亡有关(5,6)。Nur77结合到特定的DNA分子,导致靶基因调控(7)。作为一种调节细胞凋亡的可能机制,Nur77可以诱导凋亡基因的表达,如FasL和TRAIL(8,9)。 Nur77很大程度上是由多种激酶磷酸化,从而可能影响其转录活性以及其亚细胞定位(4,10,11)。Nur77从细胞核易位到线粒体,可以调节其与Bcl-2的联合,并控制细胞色素c的释放,从而引发细胞凋亡(12,13)。

Phosphorylation of Nur77 by Akt or RSK occurs at Ser351 (corresponding to rat Nur77 Ser350 and Ser354 of mouse Nur77), a site within the Nur77 DNA binding domain (14-16). Serine phosphorylation at this site can down regulate transcriptional activity of Nur77 (10,17).通过Akt或RSK的 Nur77磷酸作用发生在丝氨酸(351位)(对应大鼠Nur77的丝氨酸(350位)和小鼠Nur77的丝氨酸(354位)),该位点在Nur77 DNA结合域内(14-16)。该位点丝氨酸的磷酸化可以下调Nur77的转录活性(10,17)。

  1. Hazel, T.G. et al. (1988) Proc. Natl. Acad. Sci. USA 85, 8444-8448.
  2. Chang, C. and Kokontis, J. (1988) Biochem. Biophys. Res. Commun. 155, 971-977.
  3. Milbrandt, J. (1988) Neuron 1, 183-188.
  4. Fahrner, T.J. et al. (1990) Mol. Cell. Biol. 10, 6454-6459.
  5. Liu, Z.G. et al. (1994) Nature 367, 281-284.
  6. Woronicz, J.D. et al. (1994) Nature 367, 277-281.
  7. Wilson, T.E. et al. (1991) Science 252, 1296-1300.
  8. Weih, F. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 5533-5538.
  9. Rajpal, A. et al. (2003) EMBO J. 22, 6526-6536.
  10. Hirata, Y. et al. (1993) J. Biol. Chem. 268, 24808-24812.
  11. Hazel, T.G. et al. (1991) Mol. Cell. Biol. 11, 3239-3246.
  12. Li, H. et al. (2000) Science 289, 1159-1164.
  13. Lin, B. et al. (2004) Cell 116, 527-540.
  14. Pekarsky, Y. et al. (2001) Proc. Natl. Acad. Sci. U S A 98, 3690-3694.
  15. Han, Y.H. et al. (2006) Oncogene 25, 2974-2986.
  16. Wingate, A.D. et al. (2006) Biochem. J. 393, 715-724.
  17. Katagiri, Y. et al. (1997) J. Biol. Chem. 272, 31278-31284.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :