Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

HCFC1 Antibody (Carboxy-terminal Antigen) #50708

No. Size Price
50708S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
50708 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 100, 125, 130, 145, 260 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

HCFC1 Antibody (Carboxy-terminal Antigen) recognizes endogenous levels of total HCFC1 protein. This antibody also recognizes carboxyl terminal fragments (HCFC1-C) resulting from O-GlcNAc transferase (OGT) cleavage.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu1590 of human HCFC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HCFC1 Antibody (Carboxy-terminal Antigen).



Immunoprecipitation of HCFC1 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is HCFC1 Antibody (Carboxy-terminal Antigen). Western blot analysis was performed using HCFC1 Antibody (Carboxy-terminal Antigen).


Host cell factor C1 (HCFC1) was first identified as the host cell factor for human herpes simplex virus infection. HCFC1 and the viral protein VP16 belong to a multi-protein complex that promotes transcription of viral immediate early genes (1). The relatively large HCFC1 protein contains 6 centrally located 26 amino acid repeats that can be O-GlcNAcylated and subjected to O-linked beta-N-acetylglucosamine transferase (OGT) cleavage (2-4). The resulting amino-terminal (HCFC1-N) and carboxy-terminal (HCFC1-C) fragments are non-covalently associated and play important roles in cell cycle regulation. The HCFC1-N peptide facilitates progression through the G1 phase of the cell cycle while HCFC1-C enables proper mitosis and cytokinesis during the M phase (5-7). As HCFC1 plays an important role in neurodevelopment, mutations in the corresponding gene are associated with neurodevelopmental disorders (e.g., intellectual disability) in humans (8).

  1. Vogel, J.L. and Kristie, T.M. (2013) Viruses 5, 1272-91.
  2. Daou, S. et al. (2011) Proc Natl Acad Sci U S A 108, 2747-52.
  3. Capotosti, F. et al. (2011) Cell 144, 376-88.
  4. Lazarus, M.B. et al. (2013) Science 342, 1235-9.
  5. Julien, E. and Herr, W. (2003) EMBO J 22, 2360-9.
  6. Julien, E. and Herr, W. (2004) Mol Cell 14, 713-25.
  7. Zargar, Z. and Tyagi, S. (2012) Transcription 3, 187-92.
  8. Jolly, L.A. et al. (2015) Hum Mol Genet 24, 3335-47.

Application References

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