Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate) #5063

a-tubulin   sc-5546  

No. Size Price
5063S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
5063 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat,Monkey,D. melanogaster,Zebrafish,Bovine,Pig, Endogenous Rabbit IgG
IF-IC 1:200
CS-CA 1:50

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), CS-CA=CellSimple Cell-Assay,

Homology

Species predicted to react based on 100% sequence homology: Dog,

Specificity / Sensitivity

α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total α-tubulin protein and does not cross-react with recombinant β-tubulin.

α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate)兔单抗检测内源性α-tubulin总蛋白,但是不能与重组的β-tubulin蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the amino terminus of human α-tubulin protein.

过人工合成人源α-tubulin蛋白氨基端相应的多肽片段去免疫动物从而制备出单克隆抗体。

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated α-Tubulin (11H10) Rabbit mAb #2125.

Cell Signaling Technology抗体连接着Alexa Fluor® 488 fluorescent dye,并且直接用流式细胞仪和免疫荧光分析方法内部检测人类细胞。该抗体被期望与非连接的α-Tubulin (11H10) Rabbit mAb #2125拥有相同的物种交叉反应。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate (green). Red = propidium iodide (fluorescent DNA dye). 使用α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate (绿色)标记,共聚焦免疫荧光分析HeLa细胞。红色= propidium iodide(DNA荧光染料)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) and Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (red). 使用α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate) (绿色)和Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975标记,流式细胞仪分HeLa细胞。

Cell-based assay

Cell-based assay

CellSimple™ cell-based assay of Jurkat cells (red) using α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate) compared to a concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (grey).

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

细胞骨架由三种类型的细胞质基质纤维组成:微管、微丝(肌动蛋白纤维)和中间纤维。球形微管蛋白亚基包含微管建筑块,由α/β-tubulin异源二聚体形成所有真核细胞的tubulin亚单位。γ-tubulin被需要在tubulin亚单位的成核聚合作用中去形成微管聚合物。许多细胞运动是通过微管动力介导的,包括纤毛和鞭毛的跳动、膜泡的核质转运、在减数分裂/有丝分裂期间染色体排布和神经轴突的迁移。这些运动由于竞争性微管聚合作用和解聚作用或通过微管动力蛋白的运动(1)。

  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

CellSimple is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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