Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 555 Conjugate) #5059

a-tubulin   Alpha-tubulin ubiquitous   K-ALPHA-1 alpha tubulin {K-ALPHA-1}   sc-5546   TBAK   Tubulin alpha-ubiquitous chain   Tubulin K-alpha-1  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IF-IC 1:50 Human,Mouse,Rat,Monkey,D. melanogaster,Zebrafish,Bovine,Pig, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Dog,

Specificity / Sensitivity

α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of total α-tubulin protein. This antibody does not cross-react with recombinant β-tubulin.

α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 555 Conjugate)兔单抗检测内源性α-tubulin总蛋白,但是不能与重组的β-tubulin蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human α-tubulin. The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6.

通过人工合成人源α-tubulin蛋白氨基端相应的多肽片段去免疫动物从而制备出单克隆抗体。该抗体以F/P 比值为2-6的最佳条件连接着Alexa Fluor® 555。


This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for immunofluroescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated α-Tubulin (11H10) Rabbit mAb #2125.

Cell Signaling Technology抗体连接着Alexa Fluor® 555 fluorescent dye,并且直接用免疫荧光分析方法内部检测人类细胞。该抗体被期望与非连接的α-Tubulin (11H10) Rabbit mAb #2125有相同的物种交叉反应。



Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 555 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 555 Conjugate) (红色)标记,共聚焦免疫荧光分析HeLa细胞。蓝色= DRAQ5® #4084 (DNA荧光染料)。


The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).


  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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