Cell Signaling Technology

Product Pathways - Tag Antibodies

Cre Recombinase (D7L7L) XP® Rabbit mAb (HRP Conjugate) #50318

No. Size Price
50318S 100 µl ( 10 western blots ) ¥4,264.00 现货查询 购买询价
50318 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Transfected Only 37 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Cre Recombinase (D7L7L) Rabbit mAb (HRP Conjugate) recognizes transfected and transgenic levels of total Cre recombinase protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of bacteriophage-P1 Cre recombinase protein.

Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cre Recombinase (D7L7L) Rabbit mAb #15036.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock-transfected (-) or transfected with a construct expressing Cre recombinase (+), using Cre Recombinase (D7L7L) XP® Rabbit mAb (HRP Conjugate).

Background

Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34-base pair loxP sites. This reaction can be carried out in vitro, indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of in vitro and in vivo applications including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6).

  1. Abremski, K. et al. (1983) Cell 32, 1301-11.
  2. Sternberg, N. et al. (1981) Cold Spring Harb Symp Quant Biol 45 Pt 1, 297-309.
  3. Abremski, K. and Hoess, R. (1984) J Biol Chem 259, 1509-14.
  4. Qin, M. et al. (1994) Proc Natl Acad Sci U S A 91, 1706-10.
  5. Lakso, M. et al. (1992) Proc Natl Acad Sci U S A 89, 6232-6.
  6. Guo, F. et al. (1997) Nature 389, 40-6.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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