Cell Signaling Technology

Product Pathways - Transcription Factors

TRIM29/ATDC (E1L4E) Rabbit mAb #50292

ATDC   Trim29  

No. Size Price
50292S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
50292 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 68 Rabbit IgG
IHC-P 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

TRIM29/ATDC (E1L4E) Rabbit mAb recognizes endogenous levels of total TRIM29/ATDC protein. This antibody also cross-reacts with an unidentified protein of 49 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln499 of human TRIM29/ATDC protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin embedded BxPC-3 (left) and HeLa (right) cell pellets using TRIM29/ATDC (E1L4E) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin embedded human prostate using TRIM29/ATDC (E1L4E) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TRIM29/ATDC (E1L4E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Tripartite motif-containing protein 29 (TRIM29, ATDC) was isolated as a candidate gene by its ability to complement the radiosensitivity defect of an ataxia-telangiectasia (AT) cell line (1). This putative transcription regulator belongs to the TRIM (tripartite motif) protein family that is characterized by highly conserved amino-terminal RING finger, B-box, and coiled-coil domains. The TRIM29 protein binds and sequesters cytosolic p53, repressing expression of p53 target genes including p21 and Noxa by preventing p53 from entering the nucleus. Expression of TRIM29 inhibits p53 function and results in increased cell proliferation. (2). TRIM29 enhances tumor growth and metastasis in vivo and high TRIM29 levels are seen in most invasive pancreatic cancers. The oncogenic effect of TRIM29 appears to require β-catenin as expression of both proteins is elevated in pancreatic cancer cell lines and tissues (3).

  1. Kapp, L.N. et al. (1992) Am J Hum Genet 51, 45-54.
  2. Yuan, Z. et al. (2010) Mol Cell Biol 30, 3004-15.
  3. Wang, L. et al. (2009) Cancer Cell 15, 207-19.

Application References

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