Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Myosin IIa (Ser1943) Antibody #5026

cellular myosin heavy chain   MYH9   myosin heavy chain 9   NMHC   NMMHCA   nonmuscle myosin type A  

No. Size Price
5026S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
5026 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 230 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Myosin IIa (Ser1943) Antibody detects endogenous levels of myosin IIa protein only when phosphorylated at Ser1943.

Phospho-Myosin IIa (Ser1943) Antibody仅检测在Ser1943位点磷酸化的内源性myosin IIa蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1943 of human myosin IIa protein. Antibodies are purified using protein A and peptide affinity chromatography.

通过人工合成人源myosin IIa蛋白的Ser1943位点周围相应的磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, A-431 and 293T cells, untreated or treated with λ phosphatase and calf intestinal phosphatase (CIP), using Phospho-Myosin IIa (Ser1943) Antibody (upper) or Myosin IIa Antibody #3403 (lower).

用Phospho-Myosin IIa (Ser1943) Antibody (上图)或Myosin IIa Antibody #3403 (下图),免疫印迹(Western Blot)分析HeLa、A-431和293T细胞中Phospho-Myosin IIa和Myosin IIa蛋白水平。细胞分为λ phosphatase处理组和calf intestinal phosphatase (CIP)处理组。

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated or treated with λ phosphatase and calf intestinal phosphatase (CIP), using Phospho-Myosin IIa (Ser1943) Antibody. The antibody was pre-incubated with peptides corresponding to unphosphorylated Myosin IIa or Myosin IIa phosphorylated at Ser1943, as indicated.

使用Phospho-Myosin IIa (Ser1943) Antibody,免疫印迹(Western Blot)分析A-431细胞中Phospho-Myosin IIa(Ser1943)蛋白水平。细胞分为λ phosphatase处理组和calf intestinal phosphatase (CIP)处理组。该抗体与相应的多肽预先孵育,分别是未磷酸化的Myosin IIa多肽和在Ser1943位点磷酸化的Myosin IIa多肽,如图所示。


Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7).

非肌性myosin蛋白是一个肌动蛋白为基础的马达蛋白,其对细胞运动、细胞分裂、迁移、粘附和极化有着本质作用。全酶由两个完全相同的重链和两种轻链组成。轻链(MLCs)调节myosin II蛋白的活性和稳定。重链(NMHCs)通过三个基因MYH9、MYH10和MYH14编码,这就分别产生三种不同的非肌性myosin II亚型 IIa, IIb, and IIc(1)。当三个亚型进行同样的酶促任务时,它们结合到并收缩耦合了ATP水解作用的肌动蛋白纤维,它们的细胞内功能没有出现过剩,并且它们有不同的亚细胞分布(2-5)。myosin II蛋白羧基端尾部结构域在亚型特异性亚细胞定位中有重要作用 (6)。myosin IIa蛋白在Ser1943位点的磷酸化有助于乳腺癌细胞转移的调节(7)。

  1. Conti, M.A. and Adelstein, R.S. (2008) J Cell Sci 121, 11-18.
  2. Sandquist, J.C. et al. (2006) J Biol Chem 281, 35873-83.
  3. Even-Ram, S. et al. (2007) Nat Cell Biol 9, 299-309.
  4. Vicente-Manzanares, M. et al. (2007) J Cell Biol 176, 573-80.
  5. Wylie, S.R. and Chantler, P.D. (2008) Mol Biol Cell 19, 3956-68.
  6. Sandquist, J.C. and Means, A.R. (2008) Mol Biol Cell 19, 5156-67.
  7. Dulyaninova, N.G. et al. (2007) Mol Biol Cell 18, 3144-55.

Application References

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