Cell Signaling Technology

Product Pathways - Development

Sox2 (D6D9) XP® Rabbit mAb (ChIP Formulated) #5024

socks   stem cell  

No. Size Price
5024S 100 µl ( 10 immunoprecipitations ) ¥3,580.00 现货查询 购买询价
5024 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
ChIP 1:50 Human, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Monkey, Bovine, Dog, Horse,

Specificity / Sensitivity

Sox2 (D6D9) XP® Rabbit mAb (ChIP Formulated) detects endogenous levels of total Sox2 protein.

Sox2 (D6D9) XP® Rabbit mAb兔单抗(ChIP 级别)识别内源性的总Sox2蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Gly179 of human Sox2 protein.

单克隆抗体由合成肽段免疫动物产生,该合成肽段与邻近人源Sox2蛋白Gly179的氨基酸残基序列一致。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 10 μl of Sox2 (D6D9) XP® Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.4 x 106 NCCIT细胞染色质与SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003的10μl Sox2 (D6D9) XP®兔源单克隆抗体(ChIP级别)或2μl 正常兔源IgG#2729交联。富集到的DNA使用SimpleChIP® Human Oct-4启动子引物#4641,SimpleChIP® 人Sox2启动子引物#4649和SimpleChIP®人α卫星重复引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量通过与input染色质总量对比获得,相当于1份.4 x 106 NTERA-2细胞。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NTERA-2 cells and either 10 μl of Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) #5232, Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) #5677, Sox2 (D6D9) XP® Rabbit mAb (ChIP Formulated), or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human Nanog promoter primers, SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.NTERA-2细胞染色质与SimpleChIP® Enzymatic Chromatin IP 试剂盒(Magnetic Beads) #9003 10 μl Nanog (D73G4) XP®兔源单克隆抗体(ChIP 级别) #5232, Oct-4A (C30A3C1)兔源单克隆抗体(ChIP 级别) #5677, Sox2 (D6D9) XP® 兔源单克隆抗体(ChIP 级别),或2 μl 正常兔源IgG #2729,进行染色质免疫共沉淀。富集到的DNA使用人Nanog启动子引物,SimpleChIP® Human Oct-4启动子引物#4641,SimpleChIP® 人Nanog Sox2启动子引物#4649和SimpleChIP®人α卫星重复引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量通过与input染色质总量对比获得,相当于1份.4 x 106 NTERA-2细胞。

Background

Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3) and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8).

胚胎干细胞分离自囊泡的内细胞团,并有独特的多分化潜能和自我更新潜能。Sox2是一种对于保持细胞多潜能性起关键作用的转录因子(1)。Sox2, Oct-4和Nanog在调控网络中协同作用(1-3)。表达Sox2 或 Oct-4的siRNA诱导沉默会导致细胞多潜能性的丢失(4,5)。染色质免疫沉淀实验显示Sox2和 Oct-4能结合到数千个基因的调控位点,显示了这些转录因子在胚胎早期发育中的重要性(6,7)。最近的研究发现Sox2基因在肺癌和食管鳞癌中扩增(8).

  1. Conley, B.J. et al. (2004) Int J Biochem Cell Biol 36, 555-67.
  2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8.
  3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
  4. Boyer, L.A. et al. (2005) Cell 122, 947-56.
  5. Loh, Y.H. et al. (2006) Nat Genet 38, 431-40.
  6. Matin, M.M. et al. (2004) Stem Cells 22, 659-68.
  7. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
  8. Okita, K. et al. (2007) Nature 448, 313-7.
  9. Arnold, K. et al. (2011) Cell Stem Cell 9, 317-29.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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