Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

ASH2L (D93F6) XP® Rabbit mAb #5019

ASH2   ASH2-like protein   ASH2L1   histone methyltransferase   HMT   methyltransferase   mll   set1  

No. Size Price
5019S 100 µl ( 20 western blots ) ¥3,580.00 现货查询 购买询价
5019T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
5019 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human,Mouse,Rat,Monkey, Endogenous 80, 65 Rabbit IgG
IP 1:100
IF-IC 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: D. melanogaster,

Specificity / Sensitivity

ASH2L (D93F6) XP® Rabbit mAb detects endogenous levels of all known isoforms of the ASH2L protein.

ASH2L (D93F6) XP® Rabbit mAb兔单抗能够检测内源性ASH2L蛋白的所有已知亚型的蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ASH2L protein.

通过合成的与人源ASH2L蛋白相应的多肽片段去免疫动物从而制备出此单克隆抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ASH2L (D93F6) XP® Rabbit mAb.

使用ASH2L (D93F6) XP® Rabbit mAb兔单抗,免疫印迹(Western blot)分析不同细胞中ASH2L蛋白水平。

IF-IC

IF-IC

Confocal imunofluorescent analysis of HeLa cells using ASH2L (D93F6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

使用ASH2L (D93F6) XP® Rabbit mAb 兔单抗(绿色)标记,共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。

Background

The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, mammals contain six Set1-related proteins: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30, which are required for proper complex assembly and modulation of histone methyltransferase activity (2-6). MLL1 and MLL2 complexes contain the additional protein subunit, menin (6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis (6).

Set1组蛋白甲基转移酶蛋白首先在酵母中被鉴定,其作为Set1/COMPASS组蛋白甲基转移酶复合物的一部分,这使组蛋白H3在Lys4位点甲基化,并且作为转录的共同激活因子的功能(1)。当酵母中含有仅一种已知的Set1蛋白时,哺乳动物中含有六种Set1相关蛋白:SET1A、SET1B、MLL1、MLL2、MLL3和MLL4,所有这些蛋白组装成COMPASS样复合物以及使组蛋白H3在Lys4位点甲基化(2,3)。这些Set1相关蛋白都是在明显蛋白复合物中被发现,所有的都有共同的亚基WDR5、RBBP5、ASH2L、CXXC1和DPY30,这些对于复合物的合适装配和组蛋白甲基转移酶的活性调节是需要的(2-6)。MLL1和MLL2复合物含有额外的蛋白亚基menin (6)。像酵母Set1一样,所有六个Set1相关的哺乳类蛋白都会使组蛋白H3在Lys4位点发生甲基化(2-6)。MLL易位在许多大的血液恶性肿瘤中发现,这认为Set1/COMPASS组蛋白甲基转移酶复合物在白血病生成中起着关键作用(6)。

  1. Miller, T. et al. (2001) Proc Natl Acad Sci USA 98, 12902-7.
  2. Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
  3. Tenney, K. and Shilatifard, A. (2005) J Cell Biochem 95, 429-36.
  4. Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
  5. Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
  6. Hughes, C.M. et al. (2004) Mol Cell 13, 587-97.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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