Product Pathways - Neuroscience
CaMKII-α (6G9) Mouse mAb #50049
|50049S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|50049||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry),
Specificity / Sensitivity
CaMKII-α (6G9) Mouse mAb recognizes endogenous levels of total CaMKII-α protein. This antibody does not cross-react with CaMKII-β or CaMKII-δ proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a partially purified rat CaMKII-α protein.
Confocal immunofluorescent analysis of mouse primary neurons using CaMKII-α (6G9) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of rat brain using CaMKII-α (6G9) Mouse mAb (green). Glial cells were labeled with GFAP (D1F4Q) XP® Rabbit mAb #12389 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded mouse cerebellum using CaMKII-α (6G9) Mouse mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human CaMKII-α protein (hCaMKII-α-Myc; +), CaMKII-β protein (hCaMKII-β-Myc; +), or CaMKII-δ protein (hCaMKII-δ-Myc; +), using CaMKII-α (6G9) Mouse mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).
CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca2+/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4).
- Hughes, K. et al. (2001) J Biol Chem 276, 36008-13.
- Barria, A. et al. (1997) Science 276, 2042-5.
- Barkai, U. et al. (2000) Mol Endocrinol 14, 554-63.
- Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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