Cell Signaling Technology

Product Pathways - Neuroscience

CaMKII-α (6G9) Mouse mAb #50049

sc-32288  

No. Size Price
50049S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
50049 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 50 Mouse IgG1
IHC-P 1:1600
IF-F 1:100
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

CaMKII-α (6G9) Mouse mAb recognizes endogenous levels of total CaMKII-α protein. This antibody does not cross-react with CaMKII-β or CaMKII-δ proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a partially purified rat CaMKII-α protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of mouse primary neurons using CaMKII-α (6G9) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-F

IF-F

Confocal immunofluorescent analysis of rat brain using CaMKII-α (6G9) Mouse mAb (green). Glial cells were labeled with GFAP (D1F4Q) XP® Rabbit mAb #12389 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum using CaMKII-α (6G9) Mouse mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human CaMKII-α protein (hCaMKII-α-Myc; +), CaMKII-β protein (hCaMKII-β-Myc; +), or CaMKII-δ protein (hCaMKII-δ-Myc; +), using CaMKII-α (6G9) Mouse mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various tissues using CaMKII-α (6G9) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca2+/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4).

  1. Hughes, K. et al. (2001) J Biol Chem 276, 36008-13.
  2. Barria, A. et al. (1997) Science 276, 2042-5.
  3. Barkai, U. et al. (2000) Mol Endocrinol 14, 554-63.
  4. Makhinson, M. et al. (1999) J Neurosci 19, 2500-10.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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