Cell Signaling Technology

Product Pathways - Protein Stability

UBC3 Antibody #4997

E2   E2conjugating   UBC   Ubiquitin carrier protein   Ubiquitin protein ligase   Ubiquitin-conjugating enzyme E2-32 kD  

No. Size Price
4997S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
4997T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
4997 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 32 Rabbit
F 1:25

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry,

Specificity / Sensitivity

UBC3抗体识别内源性的UBC3和UBC3B总蛋白。

Source / Purification

UBC3多克隆抗体通过合成对应于人UBC3蛋白序列的肽段免疫动物获得,抗体经Protein A和肽亲和层析纯化。 Western blot analysis of extracts from Jurkat, CAD and C6 cells using UBC3 Antibody. 使用UBC3抗体对Jurkat、CAD和C6细胞裂解物进行免疫印迹分析的结果。 Flow cytometric analysis of untreated Jurkat cells using UBC3 antibody (blue) compared to a nonspecific negative control antibody (red). 使用UBC3抗体对未经处理的Jurkat细胞进行流式细胞仪分析的结果(蓝色),非特异性抗体(红色)作为阴性对照。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells using UBC3 antibody (blue) compared to a nonspecific negative control antibody (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat, CAD and C6 cells using UBC3 Antibody.

Background

泛素能够通过泛素化过程被共价修饰于多种细胞蛋白,导致蛋白质进入26S蛋白酶体发生降解。有三种重要成分参与靶蛋白-泛素偶联过程,泛素首先与活化酶E1形成硫酯键复合体而被激活,然后被转移到泛素运载酶E2,然后由E2转移到E3并连接到靶蛋白赖氨酸的epsilon位氨基上(1-3)。不同的E2和E3泛素酶的相互组合介导对不同底物的特异性修饰(4)。最近的研究提示,激活的E2蛋白与E3蛋白暂时偶联,两者的分离是泛素化修饰的关键步骤(5)。UBC3是酵母Cdc34基因在哺乳动物中的同源基因。UBC3B是UBC3家族成员,主要是作为E2泛素载体蛋白发挥作用。这些蛋白含有一个保守的由半胱氨酸构成的核心结构域,介导与泛素之间硫酯键的形成(6)。 UBC3与SCFSkp2(Skp1, Cullin and F-box protein/Skp2)复合体通过介导CDK 抑制因子p27的降解共同促进细胞周期由G1向S期推进(7)。UBC3B与SCFb-Trcp (Skp1, Cullin and F-box protein/b-Trcp)复合体共同介导b-catenin的降解(6)。

  1. Ciechanover, A. (1998) EMBO J. 17, 7151-7160.
  2. Hochstrasser, M. (2000) Nat. Cell Biol. 2, E153-E157.
  3. Hochstrasser, M. (2000) Science 289, 563-564.
  4. DeSalle, L.M. and Pagano, M. (2001) FEBS Lett. 490, 179-189.
  5. Deffenbaugh, A. E. et al. (2003) Cell 114, 611-622.
  6. Semplici, F. et al. (2002) Oncogene 21 , 3978-3987.
  7. Pagano, M. et al. (1995) Science 269, 682-685.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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