Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb #4991

vegf   vegf receptor   vegf receptor 2   vegf receptor-2   vegf receptor2   vegfr   vegfr 2   vegfr-2   vegfr2  

No. Size Price
4991S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
4991T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
4991 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 230 Rabbit IgG
IHC-P 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit Monoclonal Antibody detects endogenous levels of VEGF receptor 2 only when phosphorylated at Tyr951. The antibody may slightly cross-react with activated VEGF receptor 1, but not with other related tyrosine phosphorylated tyrosine kinases.

磷酸化VEGF Receptor 2 (Tyr951) (15D2) 兔单抗仅在Tyr951磷酸化时才能检测内源VEGFR2 蛋白的水平。本抗体可能与活化的VEGFR1有轻微交叉反应,但不与其它相关的酪氨酸磷酸化的酪氨酸激酶交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr951 of human VEGF receptor 2.

单克隆抗体用合成的磷酸化多肽免疫动物得到,该多肽根据人VEGF receptor 2 的Tyr951附近的氨基酸合成。

Western Blotting

Western Blotting

Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb specifically binds to phosphorylated VEGFR2, but not other phosphorylated tyrosine kinases. Western blot analysis of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho-VEGF Receptor-2 (Tyr951) (15D2) Rabbit mAb (upper) or Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (lower). CKR/PAE cells (lanes 13 and 14) express chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGF receptor-2 intracellular domain (5). CSF-1 stimulates phosphorylation of Tyr951 of intracellular VEGF receptor-2 domain (lane 13) , which was specifically detected by Phospho-VEGF Receptor-2 (Tyr951) (15D2) Rabbit mAb. Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb特异结合磷酸化的VEGFR2, 但不与其它磷酸化的酪氨酸激酶反应。用Phospho-VEGF Receptor-2 (Tyr951) (15D2) Rabbit mAb (上)或Phospho-Tyrosine mAb (P-Tyr-100) #9411 (下)对表达不同的活化的酪氨酸激酶蛋白的细胞提取物进行免疫印迹检测。CKR/PAE 细胞 (第13 和14道) 表达含有人的CSF-1胞外结合区/小鼠VEGF receptor 2胞内区的嵌合受体(5)。Phospho-VEGF Receptor-2 (Tyr951) (15D2) Rabbit mAb能特意地检测CSF-1刺激下VEGF receptor-2胞内区的Tyr951发生磷酸化 (第13道)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HUVEC cells, untreated (left) or VEGF-treated (right), using Phospho-VEGF Receptor-2 (Tyr951) (15D2) Rabbit mAb. 用Phospho-VEGF Receptor-2 (Tyr951) (15D2) Rabbit mAb 对未经处理的(左)或经过VEGF处理的(右)石蜡包埋的HUVEC细胞进行免疫组化检测。

Western Blotting

Western Blotting

Western blot analysis of recombinant human GST-VEGF Receptor 2 (Val789-Val1356), untreated or λ phosphatase-treated, using Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb (upper) and VEGF Receptor 2 Antibody # 2472 (lower). 用Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb (上) 和VEGF Receptor 2 Antibody # 2472 (下).Western blot analysis of recombinant human GST-VEGF Receptor 2 (Val789-Val1356), 对未处理的或经过λ phosphatase 处理的HUVEC进行免疫印迹检测。

Background

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).

血管内皮生长因子受体2 (VEGFR2, KDR, Flk-1)是内皮细胞上VEGF诱导信号时的一个主要受体。和配体结合之后,VEGFR2发生自磷酸化并被活化(1)。VEGFR2 主要的自磷酸化受体位于激酶插入区 (Tyr951/996)和酪氨酸激酶催化区(Tyr1054/1059) (2)。受体的激活会导致对包括Shc, Grb2, PI3K激酶, Nck 和蛋白酪氨酸磷酸化酶SHP-1 和 SHP-2接头蛋白的快速招募(3)。Tyr1212的磷酸化为Grb2提供了结合位点而且磷酸化的Tyr1175能结合PI3K激酶的p85 亚基以及PLCγ和 Shb (1,4,5)。VEGFR2信号传导对于实现VEGF刺激引起的增殖、趋化和发芽,以及体外培养的内皮细胞的增殖和体内血管的发生是必需的(6-8)。

  1. Meyer, M. et al. (1999) EMBO J 18, 363-74.
  2. Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.
  3. Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.
  4. Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
  5. Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
  6. Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
  7. Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
  8. Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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