Cell Signaling Technology

Product Pathways - NF-kB Signaling

IRF-4 Antibody #4964

ICSAT   interferon regulatory factor   irf   irf-4   IRF4   Pip   sc-6059  

No. Size Price
4964S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
4964 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 51 Rabbit
IP 1:50
ChIP 1:25

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

IRF-4 Antibody detects endogenous levels of IRF-4 protein. The antibody does not cross-react with other family members at physiological levels.

IRF-4 抗体能够检测内源性IRF-4蛋白总体水平。在生理水平上,此抗体不与其它家族成员交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Asp175 of human IRF-4. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆抗体是通过合成人源对应的IRF-4 Asp175位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos, Raji, and HuT-78 cells, using IRF-4 Antibody.Western免疫印迹。用IRF-4 Antibody研究Ramos, Raji和 HuT-78细胞的细胞提取液。

Western Blotting

Western Blotting

Western blot analysis of extracts from Karpas-620 (human) and A20 (mouse) cell lines using IRF-4 Antibody #4964 and IRF-4 (P173) Antibody #4948.Western免疫印迹。用IRF-4 Antibody #4964 和 IRF-4 (P173) Antibody #4948研究KARPAS-620 (人源)和A20 (小鼠源)细胞系的细胞提取液。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 H929 cells and either 20 μl of IRF-4 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human SUB1 Promoter Primers #5156 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.染色质免疫共沉淀。 H929细胞培养至4 x 106,用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003进行免疫沉淀实验,本实验中用20 μl IRF-4 Antibody或2 μl Normal Rabbit IgG #2729 。用SimpleChIP® Human SUB1 Promoter Primers #5156 和SimpleChIP® Human α Satellite Repeat Primers #4486对富集的DNA做real-time PCR。每个样本中沉淀的DNA量定义为相对信号与输入的总染色质相比的数值。


Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2).

干扰素调控因子(IRFs) 包含一个家族的转录调控因子,这个家族主要是在 Jak/Stat信号通路中起作用,在受到病毒的感染后能够调控干扰素(IFN)和干扰素诱导的基因表达(1)。IRFs在抵抗病原菌、自身免疫、淋巴细胞发育、细胞生长和易感性的转变等过程中发挥了重要的作用。IRF家族包含9个成员: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7和IRF-8/ICSBP。所有的IRF蛋白在N-末端的DNA-结合区域都有同源性。IRF家族成员通过与具有相似的DNA-结合区域的蛋白的反应而调控转录,例如IFN刺激的应答原件(ISRE), IFN 保守基序 (ICS) 和IFN 调控元件(IRF-E) (2)。

IRF-4 was independently cloned by three groups and demonstrated to have roles in different contexts of lymphoid regulation (3-5). First, IRF-4 (Pip) was found to associate with PU.1, a hematopoietic specific member of the ETS family, and to regulate the expression of B-cell specific genes (3). Second, it was characterized as a lymphoid-specific member of the IRF family (LSIRF) and able to bind to ISRE (4). Third, it was identified in activated T cells as a factor that binds to the promoter of the interleukin-5 gene (ICSAT), and shown to repress gene activation induced by IFN (5). IRF-4 is expressed in all stages of B cell development and in mature T cells, and is inducible in primary lymphocytes by antigen mimetic stimuli such as Concavalin A, CD3 crosslinking, anti-IgM and PMA treatment (4,5). Mice deficient in IRF-4 show normal distribution of B and T lymphocytes at 4 to 5 weeks, but later develop progressive generalized lymphadenopathy, suggesting a role for IRF-4 in the function and homeostasis of mature B- and T-lymphocytes (6).

IRF-4被3个研究组同时独立的克隆得到,并且表明其在不同的情况下对淋巴细胞的调控作用(3-5)。首先,IRF-4 (Pip)被发现与ETS家族中造血特异成员PU.1有关, 并且能特异性调控B细胞基因的表达(3)。其次,它被鉴定为一个淋巴IRF家族中特殊成员(LSIRF)能够结合到ISRE (4)。再次,它被鉴定为在激活的T细胞中作为结合到白细胞介素-5基因(ICSAT)启动子上的因子,并且在IFN的诱导下显示出对基因激活的抑制(5)。IRF-4在B细胞发育的所有阶段表达包括已经成熟度的T细胞,它在最初的淋巴细胞中能被抗原类似物如concavalin A, CD3交联, anti-IgM 和 PMA所刺激诱导(4,5)。在4-5周时,IRF-4缺失的小鼠显示了T细胞和B细胞的正常的分布,但是随后的的发育进程出现异常产生淋巴结病,这些证据表明了IRF-4在成熟的T细胞和B细胞中的作用和动态平衡(6)。

  1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
  2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
  3. Eisenbeis, C.F. et al. (1995) Genes Dev 9, 1377-87.
  4. Matsuyama, T. et al. (1995) Nucleic Acids Res 23, 2127-36.
  5. Yamagata, T. et al. (1996) Mol Cell Biol 16, 1283-94.
  6. Mittrücker, H.W. et al. (1997) Science 275, 540-3.

Application References

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