Cell Signaling Technology

Product Pathways - Tag Antibodies

Digoxigenin (D8Q9J) Rabbit mAb (HRP Conjugate) #49620

No. Size Price
49620S 100 µl ( 200 western blots ) ¥3,986.00 现货查询 购买询价
49620 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:20000 Transfected Only Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Digoxigenin (D8Q9J) Rabbit mAb (HRP Conjugate) recognizes digoxigenin conjugated molecules.

Source / Purification

Monoclonal antibody is produced by immunizing animals with KLH-conjugated digoxigenin.

Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Digoxigenin (D8Q9J) Rabbit mAb #14682.

Western Blotting

Western Blotting

Western blot analysis of digoxigenin (DIG)-conjugated BSA (0.001 ng) using Digoxigenin (D8Q9J) Rabbit mAb (HRP Conjugate).

Background

Digoxigenin (DIG) is a highly immunogenic steroid derived from “foxglove” plants of the genus Digitalis (1). This small hapten molecule can be covalently linked to proteins, nucleic acids, compounds, and glycans. Antibodies targeted against the DIG hapten display high binding affinity to DIG-conjugated molecules and can be used in molecular biology assays, including in situ hybridization, western blot, ELISA, and TUNEL assays (2-4).

  1. Cardwell, H.M. and Smith, S. (1953) Experientia 9, 367-8.
  2. Kessler, C. (1991) Mol Cell Probes 5, 161-205.
  3. Tian, F. et al. (2010) Autophagy 6, 1107-14.
  4. Lin, L. et al. (2011) J Virol Methods 171, 91-7.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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